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Protein A ELISA Kit

ELISA Kit for Protein A Detection
Catalog No. Product Name Size List Price (US$) Quantity
EK000106-9000-1 Protein A ELISA kit for detection of natural and recombinant Protein A 1 kit 650.00
Description

Protein A ELISA Kits for detection of leached Protein A are chosen by biopharmaceutical companies for their easy-to-use procedure, quick run time, sensitivity and accurate results. Our protein A ELISA kits are used in process development, process monitoring, and in final product testing to quantify natural and recombinant Protein A contamination in samples eluted off of a Protein A chromatography column.

Immunological assays are the "gold standard" for quantifying Protein A, and it is well recognized in the quality organizations that for the most accurate results the best practice is to incorporate an internal standard [and spiking protein] that is most like the contaminant ligand. For this reason we offer two distinct Protein A detection assays, each kit with its own unique standard:

* one for the detection of natural and recombinant Protein A
* another for the detection of the GE Healthcare MabSelect SureTM ligand.

The combination of a specific capture system and an optimized sample diluent yields a highly sensitive assay able to accurately detect Protein A in the presence of antibodies without the time and resources required for in-house assay development and optimization.

EK000106-9000-1: Protein A ELISA kit for detection of natural and recombinant Protein A

* Sensitivity of 0.1 ug/ml (0.8 ppm)
* Accuracy/Recovery: 80-120%
* Precision: Inter-assay 10%, Intra-assay 15%
* Specificity for all variants of natural and recombinant Protein A
* 2 hour assay completion time

Ease of Use:

* Faster sample preparation
* Minimize loss of protein
* Optional adoption, no re-validation required
* Ready-to-go "out of the box", no plate coating required
* Plug and play for process development, process monitoring, and quality control
* No individual reagent qualification
* No methods development required

The Protein A ELISA kit has been used by technology leaders in our industry for over 10 years to ensure lot-to-lot consistency in monoclonal antibody and Ig fusion products. This kit is appropriate for processes that run columns that contain any Protein A resin.

CaptivA PriMAB protein A affinity resins, which combines a 4% agarose bead with the rSPA ligand, is the major component in Opus pre-packed CaptivA PriMAB protein A columns. rSPA can be detected by our Protein A ELISA kit for detection of natural and recombinant Protein A.

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