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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal SPARC Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.
BP000038-PRO-582: Recombinant Human Secreted Protein Acidic and Rich in Cysteine (SPARC) Protein
Source: E. coli-derived.
Recombinant Human Secreted Protein Acidic and Rich in Cysteine (SPARC) Protein fused with 6X His tag is a single, non-glycosylated, polypeptide chain containing 295 amino acids and having a molecular mass of 34 kDa. Recombinant Human Secreted Protein Acidic and Rich in Cysteine (SPARC) Protein is purified by proprietary chromatographic techniques.
Purity: > 95.0% by RP-HPLC and SDS-PAGE.
Formulation: The SPARC (1 ug/ml) was lyophilized after extensive dialyses against 20 mM PBS pH 7.4.
Reconstitution: It is recommended to reconstitute the lyophilized SPARC in sterile 18 M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other SPARC Proteins:
Recombinant Human SPARC Protein
Recombinant Human SPARC Protein
SPARC Antibodies:
SPARC Polyclonal Antibody
SPARC Polyclonal Antibody
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