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SPARC Protein

Secreted Protein Acidic and Rich in Cysteine, Basement-Membrane Protein 40, BM-40, Osteonectin, ON

Catalog No. Product Name Size List Price (US$) Quantity
BP000038-C388 Recombinant Human SPARC Protein 10 ug 98.00
BP000038-C388 Recombinant Human SPARC Protein 50 ug 168.00
BP000038-C388 Recombinant Human SPARC Protein 1 mg 1056.00
Description

Introduction
Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.

BP000038-C388: Recombinant Human Secreted Protein Acidic and Rich in Cysteine (SPARC) Protein

Source: HEK 293-derived.
Recombinant Human Secreted Protein Acidic and Rich in Cysteine (SPARC) Protein is expressed with sequence (Ala18-Ile303) of Human SPARC fused with a polyhistidine tag at the C-terminus.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Other SPARC Proteins:
Recombinant Human SPARC Protein
Recombinant Human SPARC Protein

SPARC Antibodies:
SPARC Polyclonal Antibody
SPARC Polyclonal Antibody

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