PA005211-ANT-108: IL-4 Monoclonal Antibody
Rat Monoclonal Antibody;
Applications: WB, IP, ELISA, IHC, Intracellular staining;
Reactivity: Mouse;
Clone: NYRmIL-4;
Isotype: IgG1.BP000157-CYT-211: Recombinant Human Interleukin-4 (IL-4) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ug/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000157-CYT-271: Recombinant Human Interleukin-4 (IL-4) Protein, CHO
Source: Chinese Hamster Ovarian Cells-derived.
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2 ug/ml corresponding to a Specific Activity of 5-20 MIU/mg.
Purity: > 97% by SDS-PAGE and RP-HPLC.BP000157-CYT-282: Recombinant Mouse Interleukin-4 (IL-4) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ug/ml corresponding to a Specific Activity of 500,000 IU/mg.
Purity: > 96% by SDS-PAGE and RP-HPLC.BP000157-CYT-385: Recombinant Rat Interleukin-4 (IL-4) Protein
Source: E. coli-derived.
Determined by its ability to suppress LPS-induced TNF-alpha and MIP-2 production in mouse splenocytes.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000157-CYT-483: Recombinant Human Interleukin-4 (IL-4) Protein, His Tag
Source: E. coli-derived.
The ED50 for this effect is <1.8 ug/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Purity: > 95% by SDS-PAGE.BP000157-CYT-097: Recombinant Human Interleukin-4 (IL-4) Protein, HEK
Source: HEK-derived.
The activity was determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.1-0.5 ug/ml.
Purity: > 95% as obsereved by SDS-PAGE.BP005209: Human IL-4 Protein with No Tag
Source: The recombinantHuman IL-4 Protein, (amino acids His25-Ser153) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-4, No tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-4 R alpha, hFc tag with the EC50 of 9.8 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005694: Human IL-4 Protein with the C-His-Avi Tag
Source: The recombinant Human IL-4 Protein, (amino acids His25-Ser153) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-4 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-4 R alpha protein with the C-hFc tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000157-CYT-172: Recombinant Rhesus Macaque Interleukin-4 (IL-4) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependent stimulation of TF1 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0×106 IU/mg.
Purity: > 97.0% as determined by RP-HPLC and by SDS-PAGE.BP005343: Biotinylated Human IL-4 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-4 Protein, (amino acids His25-Ser153) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-4 R alpha protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-4 protein with the C-His tag with the EC50 of 11.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.