BP002456-CYT-051: Recombinant Human FAS Ligand (FASLG) Protein
Source: HEK293 cells-derived.
Fas ligand is biologically active as determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The expected ED50< 10 ng/ml, corresponding to a specific activity of 1x105 units/mg.
Purity: > 95.0% as determined by SEC-HPLC and SDS-PAGE.BP005788: Human Fas Ligand/TNFSF6 Protein with the N-His Tag
Source: The recombinant Human Fas Ligand/TNFSF6 Protein, (amino acids Pro134-Leu281) fused with a His tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Fas Ligand protein with the N-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human Fas protein with the N-hFc tag with the EC50 of 0.19 ug/ml determined by ELISA (QC Test). The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.