PA007159.h1: Recombinant Anti-DNP Monoclonal Antibody, Human IgG1 Kappa

The recombinant anti-DNP monoclonal antibody, human IgG1 (a chimeric antibody with mouse antibody variable regions and human antibody constant regions) was produced in mammalian cells.
Applications: ELISA, antigen test strip, antibody test strip, Western Blot, ICC/IF, IHC, Flow Cytometry, and ChIP.

PA007159.m2a: Recombinant Anti-DNP Monoclonal Antibody, Mouse IgG2a Kappa

The recombinant anti-DNP monoclonal antibody, mouse IgG2a (clone of DNP.m2) was produced in mammalian cells.
Applications: ELISA, antigen test strip, antibody test strip, Western Blot, ICC/IF, IHC, Flow Cytometry, and ChIP.

PA007162.mm2c: In Vivo Grade Recombinant Murinized Anti-mouse PD-1 Mouse IgG2c-L234A L235A P329G (LALAPG) Kappa Monoclonal Antibody (Clone RMP1-14.1)

The in vivo grade recombinant murinized anti-mouse PD-1 / CD279 monoclonal antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells.
Applications: immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007162.r2a: In Vivo Grade Recombinant Anti-mouse PD-1 Monoclonal Antibody (Clone RMP1-14.1), Rat IgG2a Kappa

The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibody (rat IgG2a kappa) was produced in mammalian cells. Its affintiy to the mouse PD-1 protein is <2 nM.
Applications: immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007162.m2cLA: In Vivo Grade Recombinant Anti-mouse PD-1 Monoclonal Antibody (Clone RMP1-14.1), Mouse IgG2c-L234A L235A P329G (LALAPG) Kappa

The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells.
Applications: immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.

A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.

Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.

20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.

Experienced scientists working on antibody and cell line development services for decades. >90% success rate of stable cell line generation.

Stable cell lines developed with our proprietary recombinant antibody expression system with multiple selectable markers is very productive in expressing target proteins.

Fast turnaround:

Basic service: 7-8 weeks.
Premium service: 12-14 weeks.
Pool cells: 6 weeks.

The most comprehensive hybridoma-based mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins as well as small molecular compounds; for ELISA, Western blot, IHC, and flow cytometry applications.

The lowest custom mouse monoclonal antibody production price: $11500.

Fast turnaround: 24 weeks once the antigen is available.

PA007146: In Vivo Grade Recombinant Armenian Hamster IgG1 Isotype Control Antibody

The in vivo grade recombinant hamster IgG1 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007148: In Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody

The in vivo grade recombinant hamster IgG1 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007147: In Vivo Grade Recombinant Armenian Hamster IgG2 Isotype Control Antibody

The in vivo grade recombinant Armenian hamster IgG2 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG2 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

Syd Labs has successfully developed human and monkey monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the antibody variable regions (VH and VL) from single or clonal B cells are cloned and sequenced. Recombinant rabbit monoclonal antibodies are produced, purified, and tested for various applications, such as drug development and diagnostics.

Fast turnaround: 8-10 weeks once the polyclonal sera, PBMC, and antigen is available.