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Human NKp30 Protein

CD337; LY117; NCR3; NKp30; 1C7; MALS

Catalog No. Product Name Size List Price (US$) Quantity
BP004324 Human NKp30/NCR3/CD337 Protein with the C-hFc Tag 100UG 240.00
BP004324 Human NKp30/NCR3/CD337 Protein with the C-hFc Tag 500UG 800.00
BP005548 Human NKp30/NCR3/CD337 Protein with the C-His-Avi Tag 100UG 240.00
BP005548 Human NKp30/NCR3/CD337 Protein with the C-His-Avi Tag 500UG 800.00
Description

BP004324: Human NKp30/NCR3/CD337 Protein with the C-hFc Tag

Source: The recombinant Human NKp30/NCR3/CD337 Protein, (amino acids Leu19-Thr138) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human NKp30/NCR3/CD337 protein with the hFc tag at the C-terminus has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human NKp30 protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-NKp30 Antibody protein with the C-hFc tag with the EC50 of 18.0 ng/ml determined by ELISA (QC Test). The affinity constant of 23.10 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human NKp30/NCR3/CD337 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005548: Human NKp30/NCR3/CD337 Protein with the C-His-Avi Tag

Source: The recombinant Human NKp30/NCR3/CD337 Protein, (amino acids Leu19-Thr138) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human NKp30/NCR3/CD337 protein with the His-Avi tag at the C-terminus has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-40 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human NKp30 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-NKp30 Antibody protein with the C-hFc tag with the EC50 of 2.4 ng/ml determined by ELISA. The affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human NKp30/NCR3/CD337 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human NKp30/NCR3/CD337 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following NKp Protein :
Biotinylated Human NKp30 Protein
Biotinylated Human NKp46 Protein
Cynomolgus NKp46 Protein
Human NKp30 Protein
Human NKp46 Protein
Human NKp80 Protein
Mouse NKp46 Protein
NKp46 Protein

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