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Conditions of optimal recombinant Human IL-2 R beta/CD122 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP003916: Human IL-2 R beta/CD122 Protein with the C-hFc Tag
Source: The recombinant Human IL-2 R beta/CD122 Protein, (amino acids Ala27-Asp239) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human IL-2 R beta/CD122 protein with the hFc tag at the C-terminus has a predicted MW of 51.35 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human IL-2 R beta protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-15Ra&IL-15 protein with the C-His tag with the EC50 of 18.5 ng/ml determined by ELISA (QC Test). The affinity constant of 2.88 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human IL-2 R beta/CD122 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005526: Human IL-2 R beta/CD122 Protein with the C-His-Avi Tag
Source: The recombinant Human IL-2 R beta/CD122 Protein, (amino acids Ala27-Asp239) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human IL-2 R beta/CD122 protein with the His-Avi tag at the C-terminus has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human IL-2 R beta protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-15Ra&IL-15 protein with the C-hFc tag with the EC50 of 43.7 ng/ml determined by ELISA (QC Test). The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human IL-2 R beta/CD122 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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