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Conditions of optimal recombinant Human B7-H7/HHLA2 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP004361: Human B7-H7/HHLA2 Protein with the C-hFc Tag
Source: The recombinant Human B7-H7/HHLA2 Protein, (amino acids Ile23-Asn344) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human B7-H7/HHLA2 protein with the hFc tag at the C-terminus has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD28H protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human B7-H7 protein with the C-hFc tag with the EC50 of 52.7 ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human B7-H7/HHLA2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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