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Home > Antibodies > A-Z Primary Antibodies > Biotinylated Human B7-2 Protein

Biotinylated Human B7-2 Protein

CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2

Catalog No. Product Name Size List Price (US$) Quantity
PA000732-A8076 AKT1/3 Polyclonal Antibody 100 ug 350.00
PA000732-A8076 AKT1/3 Polyclonal Antibody 50 ug 250.00
Description

BP005657: Biotinylated Human B7-2/CD86 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human B7-2/CD86 Protein, (amino acids Leu26-Pro247) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human B7-2/CD86 protein with the His-Avi tag at the C-terminus has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Biotinylated Human B7-2 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CTLA-4 protein with the C-hFc tag with the EC50 of 1.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human B7-2/CD86 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Biotinylated Human B7-2/CD86 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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