BP003182: Human IL-1R2/IL-1 RII/CD121b Protein with No Tag
Source: The recombinant Human IL-1R2/IL-1 RII/CD121b Protein, (amino acids Phe14-Glu343) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-1R2, No tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Beta, His tag with the EC50 of 0.21 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005593: Human IL-1R2/IL-1 RII/CD121b Protein with the C-His Tag
Source: The recombinant Human IL-1R2/IL-1 RII/CD121b Protein, (amino acids Phe14-Glu343) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-1R2 protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Beta protein with the C-His tag with the EC50 of 30.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005127: Human IL-1R2/IL-1 RII/CD121b Protein with the C-hFc Tag
Source: The recombinant Human IL-1R2/IL-1 RII/CD121b Protein, (amino acids Phe14-Glu343) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-1 Beta protein with the C-hFc tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-1R2 protein with the C-hFc tag with the EC50 of 0.12 ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.