Anti-Mouse CD40 Monoclonal Antibodies for Flow Cytometry

PA007315: Non-conjugated Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)

Recombinant rat IgG2a Monoclonal Antibody. 
Clone: FGK4.5 / FGK45.
Isotype:  Rat IgG2a kappa.
Source: The recombinant anti-mouse CD40 monoclonal antibody (clone: FGK4.5) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant FGK4.5 antibody specifically binds to the mouse CD40 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007316: Biotin Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)

Recombinant rat IgG2a Monoclonal Antibody with site-directed biotin conjugation through the Avi tag.
Clone: FGK4.5 / FGK45.
Isotype: Rat IgG2a kappa.
Source: The biotin anti-mouse CD40 monoclonal antibody (clone: FGK4.5) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The biotin recombinant FGK4.5 antibody specifically binds to the mouse CD40 protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The FGK4.5 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Syd Labs provides the following recombinant anti-mouse CD40 monoclonal antibodies for flow cytometry:

Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
Biotin Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
FITC Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
APC Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
PE Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
Pacific Blue Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
PerCP Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
PE/Cy5 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
PE/Cy7 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
iFluor 488 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
iFluor 549 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
iFluor 594 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
iFluor 647 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
iFluor 700 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
APC/Cy7 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)
PerCP/Cy5.5 Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5)

Background:

The recombinant FGK4.5 antibody binds to the mouse CD40 protein, a type I transmembrane protein found on antigen-presenting cells and is required for their activation. The binding of CD40L/CD154 on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. Activated CD4+ T cells primarily exhibit its ligand CD40L/CD154 to antigen-presenting cells including dendritic cells (DCs), B cells, macrophages, classical and non-classical monocytes, on a variety of non-immune cells including platelets and endothelial cells, and on several types of tumor cells.

References of anti-mouse CD40 antibody for Flow Cytometry (Clone: FGK4.5):

CD40 agonistic antibody triggers an immature DC signature in breast cancer
Mokhtari, R. B., et al. PLoS One. 2014 Apr 16;9(4):e94515. doi: 10.1371/journal.pone.0094515. PMID: 24740297
Splenocytes were stimulated with LPS (1 μg/ml) or anti-mouse CD40 antibody (FGK4.5 clone, 10 μg/ml) for 24 h to induce DC maturation. Flow cytometry analysis showed increased CD80 and CD86 expression on CD11c+ DCs after FGK4.5 stimulation. The FGK4.5 antibody induced an immature DC phenotype in tumor-bearing mice, with reduced MHC-II levels. Tumor growth was monitored weekly, revealing no significant difference in FGK4.5-treated groups. Histological examination confirmed DC infiltration in spleens post-FGK4.5 treatment.
Tags: activity of FGK4.5 antibody; function of FGK4.5 antibody

Agonistic anti-CD40 antibody-driven IL12: a novel strategy to boost DC vaccine potency
Kim, H. M., et al. J Immunol. 2012 Dec 15;189(12):6102-10. doi: 10.4049/jimmunol.1200733. PMID: 23175440
Anti-mouse CD40 mAb (clone FGK4.5) was injected intraperitoneally at 100 μg/mouse to activate DCs in vivo. Flow cytometric analysis of splenocytes demonstrated enhanced CD86 and MHC class II expression on DCs after FGK4.5 administration. IL-12 production was upregulated in FGK4.5-treated DCs, as measured by intracellular staining. Tumor challenge with B16 melanoma showed delayed growth in vaccinated mice receiving FGK4.5. Survival rates improved by 40% in the FGK4.5-boosted DC vaccine group.
Tags: FGK4.5 antibody; FGK4.5 antibody for animal model

CD40 signaling synergizes with TLR signaling in DCs to induce potent T cell immunity
Ahonen, C. L., et al. J Immunol. 2010 May 1;184(9):5039-49. doi: 10.4049/jimmunol.0903621. PMID: 20363975
Bone marrow-derived DCs were matured with LPS plus anti-mouse CD40 (FGK4.5, 5 μg/ml) for flow cytometry phenotyping. FGK4.5 stimulation synergized with TLR ligands to upregulate costimulatory molecules on DCs, confirmed by flow cytometry. Adoptive transfer of FGK4.5-matured DCs into OT-I mice enhanced antigen-specific CD8+ T cell proliferation. In vivo priming assays showed increased IFN-γ production in T cells from FGK4.5-treated groups. Multicolor flow panels included CD40, CD80, and CD86 to profile DC maturation.
Tags: FGK4.5 antibody for cancer research; FGK4.5 antibody for flow cytometry

Therapeutic antitumor response after anti-CD40 immunotherapy in B lymphoma
Houot, R., et al. Blood. 2005 Dec 1;106(12):4089-96. doi: 10.1182/blood-2005-05-2003. PMID: 16210641
Mice were treated with anti-mouse CD40 mAb (FGK4.5, 100 μg i.p.) to activate APCs in the A20 lymphoma model. Flow cytometry revealed transient B cell activation with increased CD86 expression post-FGK4.5 injection. The FGK4.5 antibody induced tumor regression in 60% of treated mice. Histopathology showed reduced lymphoma burden in lymph nodes after FGK4.5 therapy. Combination with chemotherapy enhanced the antitumor effect of FGK4.5.
Tags: FGK4.5 antibody mouse dose range; FGK4.5 mAb

In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity
Grewal, I. S., et al. J Immunol. 1997 Jan 15;158(2):721-30. doi: 10.4049/jimmunol.158.2.721. PMID: 9006927
Anti-mouse CD40 antibody (FGK4.5 clone) was used to block CD40 signaling in vivo during T-dependent responses. Flow cytometric analysis of splenocytes showed impaired germinal center B cell formation after FGK4.5 blockade. Immunoglobulin isotype switching was reduced in FGK4.5-treated mice immunized with TNP-KLH. Serum antibody titers dropped by 80% in the presence of FGK4.5. Memory B cell generation was assessed by flow cytometry, revealing defects in CD40-blocked groups.
Tags: FGK4.5 mAb for flow cytometry; FGK4.5 mAb for mouse tumor model

For more references about anti-mouse CD40 antibody for Flow Cytometry (Clone: FGK4.5), please contact our scientific support team with message@sydlabs.com.