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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: G28-5.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD40 monoclonal antibody (clone: G28-5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: G28-5) specifically binds to human CD40.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD40 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human CD40 monoclonal antibody of clone G28-5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CD4+ T Cells in the Blood of MS Patients Respond to Predicted Epitopes From B cell Receptors Found in Spinal Fluid.
H?glund, R. A., et al. Front Immunol. 2020 Apr 9;11:598. doi: 10.3389/fimmu.2020.00598. PMID: 32328067
Peripheral blood mononuclear cells from these patients were stimulated with the selected idiotope peptides in presence of anti-CD40 for 12 h. ...Cells were resuspended to a final concentration of 2.5 × 106 cells/mL, mixed with 1 ?g/mL anti-CD40 (blocking antibody, clone G28.5, BioXcell, USA) and plated onto a 96-well U bottom plate. ...500,000 PBMC were stimulated with synthetic idiotope peptides predicted to be stimulatory, tolerogenic, or inert as well as positive and negative controls for 12 h in presence of anti-CD40 antibodies. ...A total of 500,000 PBMC were left unstimulated, or stimulated with EBNA-1 peptide mix, insulin peptide mix, anti CD3/CD28 beads (not shown), or one of 22 idiotope peptides for 12 h in presence of anti-CD40 antibodies and analyzed by flow cytometry. ...In order to further characterize the idiotope peptides that elicited CD4+ T cell responses, we labeled them using metadata from the IGHV they were derived from and information on cathepsin cleavage prediction.
Tags: anti-human CD40 G28-5; anti-human CD40 G28-5 mAb
Increased B Cell ADAM10 in Allergic Patients and Th2 Prone Mice.
Cooley, L. F., et al. PLoS One. 2015 May 1;10(5):e0124331. doi: 10.1371/journal.pone.0124331. PMID: 25933166
B cells were cultured for 2 days (or 5 days for CD23 ELISA) with 200 ng/mL human IL-21 (ATCC), 1 ?g/mL anti-CD40 (Clone G28-5, ATCC), and 10 ng/mL rhIL-4 (R&D). ...Positively selected B220+ splenic B cells (Miltenyi Biotec) were grown for 1?3 days in cultures containing 1000 units IL-4 and 50 ?g/mL LPS (E. coli 0111:B4, Sigma) or 1.25?g/mL purified anti-mouse CD40 (Biolegend). ...Our results thus far indicate that Th2 prone mice and humans, whose B cells are known to produce excess IgE, exhibit increased B cell ADAM10 even in a na?ve, non-stimulated state. After stimulation with anti-CD40/IL4, B-ADAM10 levels increased more dramatically in Th2 prone strain B cells as well. ...T cell help as demonstrated by in vitro culture with equal concentrations of anti-CD40/IL4 shows that B cell ADAM10 increases in both C57 and Balb WT B cells following stimulation. ...Importantly, however, Balb WT B cells exhibit increased ADAM10 in the na?ve state and even more dramatically increased ADAM10 after anti-CD40/IL4 stimulation compared C57-WT B cells.
Tags: anti-human CD40 G28-5 antibody in vivo; anti-human CD40 G28-5 in animal model
Analysis of Epstein-Barr virus-regulated host gene expression changes through primary B-cell outgrowth reveals delayed kinetics of latent membrane protein 1-mediated NF-?B activation.
Price, A. M., et al. J Virol. 2012 Oct;86(20):11096-106. doi: 10.1128/JVI.01069-12. PMID: 22855490
An agonistic anti-CD40 antibody was purified from the supernatant of the G28-5 hybridoma cell line (a kind gift of E. Kieff, Harvard Medical School). ...IKK-2 inhibitor IV (1 ?M) or the agonistic CD40 antibody G28-5 (1 ?g/ml) was added to the cells at the various time points described. ...We specifically asked whether EBV-induced B-cell outgrowth would be affected by CD40 activation by using the agonistic antibody G28-5 during 1- or 2-week intervals following EBV infection of primary B cells. ...Therefore, we performed experiments similar to those described above for CD40 agonism, but using a specific inhibitor of the IKK? subunit of the IKK complex. ...We thank Elliott Kieff (Harvard Medical School) for the G28-5 (anti-CD40) hybridoma and Thomas Tedder (Duke University) for the 33-6-6 (anti-CD19) hybridoma.
Tags: anti-human CD40 G28-5 mAb in animal model; anti-human CD40 G28-5 in cancer research
For more references about Anti-human CD40 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-mouse CD40 monoclonal antibodies:
In vivo grade recombinant anti-mouse CD40 monoclonal antibodies (clone FGK4.5)
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