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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: G28-5.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD40 monoclonal antibody (clone: G28-5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: G28-5) specifically binds to human CD40.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD40 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human CD40 monoclonal antibody of clone G28-5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
A bispecific CD40 agonistic antibody allowing for antibody-peptide conjugate formation to enable cancer-specific peptide delivery, resulting in improved T Cell proliferation and anti-tumor immunity in mice
Vangrevelynghe E, et al. Nat Commun. 2024 Nov 5;15(1):9500. doi: 10.1038/s41467-024-53839-5. PMID: 39551234
The bispecific CD40 agonistic antibody BiA9*2_HF, based on G28-5, delivered antigenic peptides with simultaneous CD40 stimulation, enhancing T cell expansion in vivo. In mice, it induced 10?15-fold CD8+ and 2?7-fold CD4+ T cell proliferation in draining lymph nodes, significantly improving anti-tumor responses compared to controls.
Tags: anti-human CD40 G28-5; anti-human CD40 G28-5 mAb
CD40-activated B cells induce anti-tumor immunity in vivo
Hoffmann P, et al. Oncoimmunology. 2016 Jul 3;5(8):e1199294. doi: 10.1080/2162402X.2016.1199294. PMID: 26934557
CD40-activated B cells, stimulated via the G28-5 antibody, induced anti-tumor immunity in vivo in luciferase+ mice. Expression of homing molecules CXCR4, CXCR5, CCR7, and CD62L was upregulated in CD40B cells by day 14, enhancing their role in combinatorial immunotherapies with checkpoint inhibitors.
Tags: anti-human CD40 G28-5 antibody in vivo; anti-human CD40 G28-5 in animal model
Combination of an agonistic anti-CD40 monoclonal antibody and the COX-2 inhibitor celecoxib induces anti-glioma effects by promotion of type-1 immunity in myeloid cells and T-cells
Antonia SJ, et al. Cancer Immunol Immunother. 2014 Oct;63(10):979-91. doi: 10.1007/s00262-014-1570-4. PMID: 24878890
The G28-5 agonistic CD40 monoclonal antibody, combined with celecoxib, activated myeloid cells and promoted anti-tumor immunity in C57BL/6 mice bearing Quad-GL261 or Sleeping Beauty gliomas. Brain-infiltrating lymphocytes (BILs), analyzed by flow cytometry on day 5 post-treatment, confirmed enhanced type-1 immunity.
Tags: anti-human CD40 G28-5 mAb in animal model; anti-human CD40 G28-5 in cancer research
Agonistic and antagonistic properties of CD40 mAb G28-5 are dependent on binding valency
Fuleihan R, et al. J Immunol. 1995 May 1;154(9):4240-6. doi: 10.4049/jimmunol.154.9.4240. PMID: 7743602
The G28-5 monoclonal antibody triggered partial CD40 responses on B lymphocytes while acting as a partial antagonist by preventing gp39 binding. Fab fragments of G28-5 retained inhibitory activity but lost crosslinking-dependent stimulatory function, highlighting valency-dependent effects.
Tags: anti-human CD40 G28-5 mAb in cancer research; anti-human CD40 G28-5 mAb
In vivo efficacy and toxicity of a single-chain immunotoxin targeted to CD40
Francisco JA, et al. Blood. 1997 Jun 15;89(12):4493-500. doi: 10.1182/blood.V89.12.4493. PMID: 9192773
The G28-5 sFv-PE40 single-chain immunotoxin, targeting CD40 on B-cell malignancies, had a binding affinity of 3 nmol/L, within 15-fold of the bivalent G28-5 antibody. In lymphoma xenografted mice, it induced complete antitumor responses at well-tolerated doses, showing efficacy in mice, rats, and monkeys.
Tags: bioactivity of anti-human CD40 G28-5; anti-human CD40 G28-5 of low endotoxin
For more references about anti-human CD40 antibody (G28-5), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-mouse CD40 monoclonal antibodies:
In vivo grade recombinant anti-mouse CD40 monoclonal antibodies (clone FGK4.5)
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