BP004266: Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FMNKFIYEI peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody protein with the C-hFc tag with the EC50 of 1.9 ng/ml determined by ELISA (QC Test). The affinity constant of 0.923 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003838: Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein, (amino acids Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI ) Monomer protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody protein with the C-hFc tag with the EC50 of 2.7 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.