BP005005: Biotinylated Human IL-5 Protein with the N-His-Avi Tag
Source: The recombinant Biotinylated Human IL-5 Protein, (amino acids Ile20-Ser134) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-5 R alpha protein with the N-His-Avi tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-5 protein with the N-His tag with the EC50 of 90.4 ng/ml determined by ELISA (QC Test). The affinity constant of 7.57 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
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