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Conditions of optimal DARPP-32 (Phosphorylated) antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
DARPP-32 is localized to neurons containing dopamine receptors and is a potent inhibitor of protein phosphatase 1, which plays a central role in dopaminergic and glutamatergic signaling, and in integrating the activity of these two pathways. Dopamine, acting through the D1 receptor and the adenylate cyclase-stimulatory G protein Gs/Golf, activates adenylate cyclase, thereby bringing about protein kinase A (PKA)-mediated phosphorylation of DARPP-32. By contrast, acting through the D2 receptor and the adenylate cyclase-inhibitory Gi proteins, dopamine reduces DARPP-32 phosphorylation. Calcineurin acts as a principle mediator of dephosphorylation-dependent inactivation of DARPP-32 (see ref. 5 for a review). Consistent with these observations, knockout mice with disruption of the DARPP-32 gene have been previously shown to have alterations in their molecular, electrophysiological, and behavioral responses to dopamine, drugs of abuse, and antipsychotic medications.
PA000438-A7056: DARPP-32 (Phospho-Thr75) Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.
Applications: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:20000.
Reactivity: Human, Mouse, Rat.
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 34 (R-P-TP-P-A).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
Other DARPP-32 (Phosphorylated) Antibody:
DARPP-32(Phospho-Thr75)Polyclonal Antibody
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