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Conditions of optimal recombinant cynomolgus PD-L1/B7-H1 protein performance should be determined experimentally by the investigator. A promotion of 20% discount (coupon code PDL1protein24A20) is effective until June 30, 2024.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
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BP005427: Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant cynomolgus/rhesus macaque PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant cynomolgus/rhesus macaque PD-L1/B7-H1 protein with the C-His tag has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the anti-cynomolgus PD-L1 antibody with the hFc tag to the immobilized cynomolgus/rhesus macaque PD-L1/B7-H1 protein with the His tag at 1 ug/ml (100 ul/Well) was 6.2 ng/ml determined by ELISA. The recombinant cynomolgus PD-1 protein with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant cynomolgus/rhesus macaque PD-L1 protein with the C-His tag with an affinity constant of 1.28 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant cynomolgus/rhesus macaque PD-L1/B7-H1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005273: Cynomolgus PD-L1/B7-H1 Protein with the C-hFc Tag
Source: The recombinant cynomolgus PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant cynomolgus PD-L1/B7-H1 protein with the C-hFc tag has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the biotinylated anti-cynomolgus PD-L1 antibody with the hFc tag to the immobilized cynomolgus PD-L1/B7-H1 protein with the hFc tag at 1 ug/ml (100 ul/Well) was 10.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant cynomolgus PD-L1/B7-H1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant cynomolgus PD-L1/B7-H1 protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
PD1 and PDL1 Antibodies:
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
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