BP005222: Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein with the C-His-Avi Tag
Source: The recombinant Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, (amino acids Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-HLA-G Antibody protein with the C-hFc tag with the EC50 of 6.2 ng/ml determined by ELISA (QC Test). The affinity constant of 4.62 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005221: Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, (amino acids Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-HLA-G Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer protein with the C-His tag with the EC50 of 19.9 ng/ml determined by ELISA (QC Test). The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003278: Human HRG/HPRG Protein with the C-His Tag
Source: The recombinant Human HRG/HPRG Protein, (amino acids Val19-Lys525) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005720: Biotinylated Human TRAIL R4/TNFRSF10D Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human TRAIL R4/TNFRSF10D Protein, (amino acids Ala56-His211) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human TRAIL protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human TRAIL R4 protein with the C-His tag with the EC50 of 0.14 ug/ml determined by ELISA (QC Test). The affinity constant of 0.135 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.