BP000615-PKA-226: Recombinant Human Hexokinase-1
Source: E. coli-derived.
Specific activity is 7-8 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C.
Purity > 90%, by RP-HPLC and SDS-PAGE.
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