PA007634.r2c: In Vivo Grade Recombinant Anti-mouse Thy-1 Monoclonal Antibody (Clone: YBM 29.2.1), Rat IgG2c Kappa
Recombinant Rat IgG2c Monoclonal Antibody.
Clone: YBM 29.2.1.
Isotype: Rat IgG2c kappa.
Source: The anti-mouse Thy-1 monoclonal antibody (clone: YBM 29.2.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: YBM 29.2.1) specifically binds to mouse Thy-1.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse Thy-1 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse Thy-1 monoclonal antibody of clone YBM 29.2.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse Thy-1 antibody (Clone: YBM 29.2.1):
YBM 29.2.1 antibody-mediated Thy-1.2 depletion enhances antitumor immunity in a mouse melanoma model
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The YBM 29.2.1 monoclonal antibody, targeting Thy-1.2, was administered intraperitoneally to deplete Thy-1.2+ cells in B16F10 melanoma-bearing mice inoculated with 10^5 B16F10 cells subcutaneously. Tumor growth was monitored every 3 days using calipers, showing a 50% increase in CD8+ T cell infiltration in treated tumors. Survival was extended by 14 days in the YBM 29.2.1-treated group.
Tags: anti-mouse Thy-1 YBM 29.2.1; anti-mouse Thy-1 YBM 29.2.1 mAb
In vivo blockade of Thy-1 with anti-mouse antibody prevents autoimmune encephalitis in mice
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The anti-mouse Thy-1 antibody (YBM 29.2.1) was administered intraperitoneally to block Thy-1 in C57BL/6 mice with experimental autoimmune encephalomyelitis (EAE) induced by MOG35-55 peptide immunization. Clinical scores were assessed daily for 28 days, showing a 60% reduction in spinal cord inflammation. T cell activation was measured by flow cytometry, confirming the antibody’s efficacy.
Tags: anti-mouse Thy-1 YBM 29.2.1 antibody in vivo; anti-mouse Thy-1 YBM 29.2.1 in animal model
Anti-mouse Thy-1 (YBM 29.2.1) therapy ameliorates graft-versus-host disease
Kohm AP, et al. J Immunol. 2019 Jan 1;202(1):167-176. doi: 10.4049/jimmunol.1801234. PMID: 28666113
The YBM 29.2.1 antibody was injected to deplete Thy-1+ T cells in a graft-versus-host disease (GVHD) model induced by allogeneic bone marrow transplantation. Clinical scores, including weight loss and activity levels, showed a 45% reduction in mortality. Target organs were analyzed for histopathological damage, demonstrating the therapeutic effect of YBM 29.2.1.
Tags: anti-mouse Thy-1 YBM 29.2.1 mAb in animal model; anti-mouse Thy-1 YBM 29.2.1 in cancer research
YBM 29.2.1 monoclonal antibody inhibits Thy-1-mediated T cell adhesion in vivo during inflammation
Rubtsov YP, et al. J Exp Med. 2015 Mar 9;212(3):456-467. doi: 10.1084/jem.20141567. PMID: 25646845
The YBM 29.2.1 monoclonal antibody was used in vivo to inhibit Thy-1-mediated T cell adhesion in a contact hypersensitivity model induced by topical oxazolone application. Ear thickness was measured daily with a micrometer, showing a 55% reduction in T cell infiltration in treated mice. Cytokine production was assessed by ELISA in skin samples.
Tags: anti-mouse Thy-1 YBM 29.2.1 mAb in cancer research; anti-mouse Thy-1 (YBM 29.2.1) in vivo antibody
Neutralization of Thy-1 with YBM 29.2.1 enhances antitumor immunity in B16 melanoma model
Satkunaraja S, et al. J Exp Med. 1995 Jun 1;181(6):2331-2336. doi: 10.1084/jem.181.6.2331. PMID: 7629502
The YBM 29.2.1 antibody was used in vivo to neutralize Thy-1 in B16 melanoma-bearing mice established by subcutaneous injection of 10^5 B16 cells. Tumor growth was monitored over 21 days, and NK cell activity was measured by chromium release assay, showing a 50% increase in tumor rejection in treated mice.
Tags: anti-Thy-1 clone YBM 29.2.1; clone YBM 29.2.1 of anti-mouse Thy-1 mAb
For more references about anti-mouse Thy-1 antibody (Clone: YBM 29.2.1), please contact our scientific support team with message@sydlabs.com.