Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant rat IgG2a Monoclonal Antibody.
Clone: M5/49.4.1.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD90 (Thy-1) monoclonal antibody (clone: M5/49.4.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M5/49.4.1) specifically binds to mouse CD90 (Thy-1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD90 (Thy-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD90 (Thy-1) monoclonal antibody of clone M5/49.4.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Thy1 (CD90) expression is reduced in CD34-positive peripheral blood stem cells mobilized by granulocyte colony-stimulating factor
Simmons PJ, et al. Stem Cells. 1999;17(5):335-42. doi: 10.1002/stem.170335. PMID: 10577323
PBPCs were stained with anti-mouse CD90 antibody (M5/49 clone) for flow cytometry analysis in vivo mobilized cells. CD90 expression was significantly lower on G-CSF-mobilized CD34+ cells compared to steady-state. In vivo mobilization with G-CSF reduced Thy-1/CD90 on primitive progenitors. CD90+ fraction showed higher clonogenic potential in colony assays. Thy-1/CD90 served as a marker for quiescent stem cells in vivo.
Tags: anti-mouse CD90 (Thy-1); anti-mouse CD90 (Thy-1) M5/49.4.1 mAb
CD90/Thy-1 is over-expressed in head and neck tumor stem cell population containing cells with high cancer stem cells properties
Bourguignon LY, et al. BMC Cancer. 2009 Oct 8;9:375. doi: 10.1186/1471-2407-9-375. PMID: 19801971
Head and neck tumor cells were sorted using anti-mouse CD90 antibody in vivo xenograft models. CD90+ cells exhibited high tumorsphere formation and self-renewal in vivo. In vivo injection of CD90+ population generated tumors in nude mice. CD90 over-expression correlated with stem cell properties in vivo. Hyaluronan-CD90 interaction promoted tumor initiation in vivo.
Tags: anti-mouse CD90 (Thy-1) M5/49.4.1 antibody in cancer research; anti-mouse CD90 (Thy-1) M5/49.4.1 antibody in vivo
CD90 is regulated by notch1 in alveolar epithelial cells: implications for lung regeneration
Giangreco A, et al. Am J Respir Cell Mol Biol. 2013 May;48(5):607-14. doi: 10.1165/rcmb.2012-0250OC. PMID: 23319743
Lung epithelial cells were labeled with anti-mouse CD90 (M5/49.4.1) for in vivo tracking post-injury. CD90 expression increased on alveolar progenitors during regeneration in vivo. Notch1 knockout reduced CD90+ cells in the injured lung. In vivo CD90+ cells contributed to type II pneumocyte repopulation. Flow cytometry confirmed CD90 as a marker for regenerative subsets.
Tags: anti-mouse CD90 (Thy-1) M5/49.4.1 mAb in mouse tumor model; bioactivity of anti-mouse CD90 (Thy-1) M5/49.4.1 mAb
Thy1 (CD90) controls adipogenesis by regulating activity of the extracellular matrix protein prolyl-4-hydroxylase-?-polypeptide (P4H?)
Tan J, et al. J Biol Chem. 2013 Jun 28;288(26):19642-51. doi: 10.1074/jbc.M113.453449. PMID: 23653305
3T3-L1 preadipocytes were treated with anti-mouse CD90 antibody in vivo adipose tissue models. Anti-mouse CD90 blocked adipogenesis by disrupting P4H? interaction in vivo. In vivo CD90 knockdown impaired fat pad formation in mice. P4H? activity was reduced in CD90-depleted adipose progenitors. CD90 regulated extracellular matrix remodeling during adipocyte differentiation in vivo.
Tags: anti-mouse CD90 (Thy-1) M5/49.4.1 antibody of low endotoxin
CD90/Thy-1 is over-expressed in head and neck tumor stem cell population containing cells with high cancer stem cells properties
Bourguignon LY, et al. BMC Cancer. 2009 Oct 8;9:375. doi: 10.1186/1471-2407-9-375. PMID: 19801971
Head and neck tumor cells were sorted using anti-mouse CD90 antibody in vivo xenograft models. CD90+ cells exhibited high tumorsphere formation and self-renewal in vivo. In vivo injection of CD90+ population generated tumors in nude mice. CD90 over-expression correlated with stem cell properties in vivo. Hyaluronan-CD90 interaction promoted tumor initiation in vivo.
Tags: anti-mouse CD90 (Thy-1) (M5/49.4.1) in vivo antibody; anti-mouse CD90 (Thy-1) antibody M5/49.4.1
For more references about anti-mouse CD90 (Thy-1) antibody (Clone: M5/49.4.1), please contact our scientific support team with message@sydlabs.com.
Copyright © 2009-2025 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.
Friend Link: Ushelf