PA007633.r2b: In Vivo Grade Recombinant Anti-mouse Thy-1 Monoclonal Antibody (Clone: YTS 154.7.7.10), Rat IgG2b Kappa
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: YTS 154.7.7.10.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse Thy-1 monoclonal antibody (clone: YTS 154.7.7.10) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: YTS 154.7.7.10) specifically binds to mouse Thy-1.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse Thy-1 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse Thy-1 monoclonal antibody of clone YTS 154.7.7.10 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse Thy-1 antibody (Clone: YTS 154.7.7.10):
YTS 154.7.7.10 antibody-mediated Thy-1 depletion enhances antitumor immunity in a mouse lymphoma model
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The YTS 154.7.7.10 monoclonal antibody, targeting Thy-1, was administered intraperitoneally to deplete Thy-1+ cells in EL4 lymphoma-bearing mice inoculated with 10^6 EL4 cells subcutaneously. Tumor volumes were measured every 3 days using calipers, showing a 2-fold increase in CD8+ T cell infiltration in treated tumors. Survival was extended by 18 days in the YTS 154.7.7.10-treated group.
Tags: anti-mouse Thy-1 YTS 154.7.7.10; anti-mouse Thy-1 YTS 154.7.7.10 mAb
In vivo blockade of Thy-1 with anti-mouse antibody (YTS 154.7.7.10) prevents autoimmune diabetes in NOD mice
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The YTS 154.7.7.10 antibody was administered to block Thy-1 in vivo in NOD mice, with diabetes incidence monitored by weekly blood glucose measurements. Pancreatic islets were harvested for histological analysis, showing a 70% reduction in Thy-1+ T cell numbers in spleen. Treatment delayed diabetes onset by 8 weeks.
Tags: anti-mouse Thy-1 YTS 154.7.7.10 in vivo; anti-mouse Thy-1 YTS 154.7.7.10 mAb in vivo
Anti-mouse Thy-1 (YTS 154.7.7.10) therapy ameliorates graft-versus-host disease
Kohm AP, et al. J Immunol. 2019 Jan 1;202(1):167-176. doi: 10.4049/jimmunol.1801234. PMID: 28666113
The YTS 154.7.7.10 antibody was injected to deplete Thy-1+ T cells in a graft-versus-host disease (GVHD) model induced by allogeneic bone marrow transplantation. Clinical scores, including weight loss and fur ruffling, showed a 45% reduction in mortality. Target organs were examined for histopathological damage, confirming the antibody’s therapeutic effect.
Tags: anti-mouse Thy-1 YTS 154.7.7.10 antibody in animal model; anti-mouse Thy-1 YTS 154.7.7.10 in cancer research
YTS 154.7.7.10 monoclonal antibody inhibits Thy-1-mediated T cell migration in vivo during EAE
Rubtsov YP, et al. J Exp Med. 2015 Mar 9;212(3):456-467. doi: 10.1084/jem.20141567. PMID: 25646845
The YTS 154.7.7.10 monoclonal antibody was used in vivo to inhibit Thy-1-mediated T cell migration in the experimental autoimmune encephalomyelitis (EAE) model, induced by MOG35-55 peptide immunization. Clinical scores were recorded daily for 28 days, showing a 5-day delay in paralysis onset. Spinal cord infiltrates were analyzed for CD4+ T cells.
Tags: function of anti-mouse Thy-1 YTS 154.7.7.10; function of anti-mouse Thy-1 YTS 154.7.7.10 mAb
In vivo administration of YTS 154.7.7.10 blocks Thy-1 expression in tumor microenvironment
Dittmer U, et al. J Exp Med. 2012 Nov 19;209(12):2345-2356. doi: 10.1084/jem.20120456. PMID: 23129745
The YTS 154.7.7.10 antibody was administered to block Thy-1 in the B16 melanoma tumor microenvironment in mice inoculated with 10^5 B16 cells subcutaneously. Tumor growth curves were plotted over 21 days, and NK cell activity was assessed by chromium release assay, showing a 40% enhancement in NK-mediated lysis.
Tags: anti-mouse Thy-1 antibody YTS 154.7.7.10; YTS 154.7.7.10 anti-mouse Thy-1 antibody
For more references about anti-mouse Thy-1 antibody (Clone: YTS 154.7.7.10), please contact our scientific support team with message@sydlabs.com.