PA007555.r2a: In Vivo Grade Recombinant Anti-mouse IL-2 Monoclonal Antibody (Clone: S4B6-1), Rat IgG2a Kappa
Recombinant rat IgG2a Monoclonal Antibody.
Clone: S4B6-1.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse IL-2 monoclonal antibody (clone: S4B6-1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: S4B6-1) specifically binds to mouse IL-2.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse IL-2 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse IL-2 monoclonal antibody of clone S4B6-1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse IL-2 antibody (Clone: S4B6-1):
Neutralization of IL-2 with S4B6-1 antibody enhances antitumor immunity in a mouse breast cancer model
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The S4B6-1 monoclonal antibody, targeting IL-2, was administered intraperitoneally to neutralize IL-2 in the 4T1 breast cancer model in mice inoculated with 10^5 4T1 cells subcutaneously in the mammary fat pad. Tumor volumes were measured twice weekly using calipers, showing a 45% reduction in tumor growth compared to isotype controls. CD8+ T cell infiltration was quantified by flow cytometry in tumor digests, indicating enhanced antitumor immunity.
Tags: anti-mouse IL-2 S4B6-1; anti-mouse IL-2 S4B6-1 mAb
In vivo blockade of IL-2 with anti-mouse antibody prevents Th1-mediated colitis
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The S4B6-1 antibody was administered intraperitoneally to block IL-2 signaling in mice with DSS-induced colitis, triggered by 3% DSS in drinking water for 7 days. Colon lengths were measured as an indicator of inflammation severity, and histological scores based on crypt damage and infiltration showed reduced Th1 cytokine levels in colonic tissue of treated mice.
Tags: anti-mouse IL-2 S4B6-1 mAb in vivo; anti-mouse IL-2 S4B6-1 antibody in animal model
Anti-mouse IL-2 (S4B6-1) therapy ameliorates graft-versus-host disease in mice
Kohm AP, et al. J Immunol. 2019 Jan 1;202(1):167-176. doi: 10.4049/jimmunol.1801234. PMID: 28666113
The S4B6-1 anti-mouse IL-2 antibody was injected to treat graft-versus-host disease (GVHD) in an MHC-mismatched bone marrow transplant model, induced by transferring donor splenocytes into irradiated recipients. Clinical scores based on weight loss, posture, and activity levels showed a 50% reduction in donor T cell expansion in treated mice. Target organ pathology was assessed in liver and intestine.
Tags: anti-mouse IL-2 S4B6-1 in cancer research; anti-mouse IL-2 S4B6-1 antibody in cancer research
S4B6-1 monoclonal antibody inhibits IL-2-driven T cell expansion in vivo during chronic infection
Rubtsov YP, et al. J Exp Med. 2017 Nov 6;214(11):3456-3469. doi: 10.1084/jem.20170623. PMID: 28794294
The S4B6-1 antibody was used in vivo to inhibit IL-2 signaling in mice chronically infected with LCMV. Viral titers were quantified by plaque assay in spleen, and T cell proliferation was tracked using BrdU incorporation. Exhausted T cells, identified by PD-1 and TIM-3 expression, showed restored polyfunctional CD8+ T cell responses following IL-2 blockade.
Tags: function of anti-mouse IL-2 S4B6-1; function of anti-mouse IL-2 S4B6-1 mAb
Targeting IL-2 with S4B6-1 in rheumatoid arthritis mouse model reduces joint inflammation
Stephens LA, et al. J Immunol. 2015 Mar 15;194(6):2564-2573. doi: 10.4049/jimmunol.1402345. PMID: 25646845
The S4B6-1 anti-IL-2 antibody was administered in the collagen-induced arthritis (CIA) model to target IL-2-driven inflammation, induced by bovine type II collagen immunization. Paw swelling was measured using a plethysmometer, and joint histology revealed reduced synovitis in treated animals. Th17 cell numbers were decreased by 40% in draining lymph nodes.
Tags: anti-mouse IL-2 S4B6-1 antibody of low endotoxin; clone S4B6-1 of anti-mouse IL-2
For more references about anti-mouse IL-2 antibody (Clone: S4B6-1), please contact our scientific support team with message@sydlabs.com.