Anti-Mouse IL-2 Monoclonal Antibodies

PA007555.r2a: In Vivo Grade Recombinant Anti-mouse IL-2 Monoclonal Antibody (Clone: S4B6-1), Rat IgG2a Kappa

Recombinant rat IgG2a Monoclonal Antibody.
Clone: S4B6-1.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse IL-2 monoclonal antibody (clone: S4B6-1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: S4B6-1) specifically binds to mouse IL-2.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse IL-2 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse IL-2 monoclonal antibody of clone S4B6-1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse IL-2 Monoclonal Antibody:

GITR intrinsically sustains early type 1 and late follicular helper CD4 T cell accumulation to control a chronic viral infection.
Clouthier, D. L., et al. PLoS Pathog. 2015 Jan;11(1):e1004517. doi: 10.1371/journal.ppat.1004517. PMID: 25590581
GITR is dispensable for initial CD4 T cell proliferation and differentiation, but supports the post-priming accumulation of IFN?+IL-2+ Th1 cells, facilitating CD8 T cell expansion and early viral control. ... Consistently, we pinpoint IL-2-dependent CD4 T cell help for CD8 T cells to between days four and eight p.i. ... IL-2 is important for CD8 T cell accumulation and function in the context of chronic LCMV infection. ... IL-2 blockade reduced the LCMV-specific Th1 and CD8 T cell responses in GITR+/+ mice to the level observed in GITR-/- mice, whereas IL-2 blockade had no impact on the T cell responses in GITR-/- mice. ... There was also a consistent trend toward fewer GITR- IL-2+ IFN?+ SMARTA cells relative to the GITR+ SMARTA.
Tags: anti-mouse IL-2 S4B6-1; anti-mouse IL-2 S4B6-1 mAb

Activated regulatory T cells suppress effector NK cell responses by an IL-2-mediated mechanism during an acute retroviral infection.
Littwitz-Salomon, E., et al. Retrovirology. 2015 Jul 30;12:66. doi: 10.1186/s12977-015-0191-3. PMID: 26220086
Suppression of NK cell functions depended on IL-2 consumption by Tregs, which could be overcome by specific NK cell stimulation with an IL-2/anti-IL-2 mAb complex. ... Suppression by Tregs, which was mediated by IL-2 consumption, led to increased viral loads and reduced cytotoxicity of NK cells in vivo. ... To confirm that IL-2 is an important factor in Treg-mediated suppression of NK cell responses, we specifically stimulated NK cells with IL-2 using a complex of anti-mouse IL-2 mAb (clone S4B6-1) in combination with recombinant mouse IL-2 protein. ... Here, we describe a new suppressive mechanism of Tregs on NK cell functions during an acute viral infection that is dependent on IL-2 availability or IL-2 consumption by Tregs. ... If indicated, Tregs were depleted by repeated injections of DT and mice were stimulated by injections of IL-2/anti-IL-2 mAb complex.
Tags: anti-mouse IL-2 S4B6-1 mAb in vivo; anti-mouse IL-2 S4B6-1 antibody in animal model

Type I interferons directly inhibit regulatory T cells to allow optimal antiviral T cell responses during acute LCMV infection.
Srivastava, S., et al. J Exp Med. 2014 May 5;211(5):961-74. doi: 10.1084/jem.20131556. PMID: 24711580
IL-2 is produced by activated pathogen-specific CD4+ T cells (Long and Adler, 2006), and recognition of pathogen-associated molecular patterns drives dendritic cell activation, resulting in increased antigen presentation and expression of MHC class II and co-stimulatory ligands. ... However, pretreatment of T reg cells for 30 min with IFN-? did not affect IL-2?induced phosphorylation of STAT5 (unpublished data). ... This inhibition was not secondary to impaired IL-2 signaling caused by reduced activation of effector T cells, as STAT5 phosphorylation in T reg cells was not decreased after B7-1/B7-2 blockade (unpublished data). ... That T reg cells and effector T cells both rely on similar signals?like IL-2 and TCR/coreceptor signaling?for their activation and proliferation has made it difficult to understand how these functionally opposed cell types are differentially regulated. ... In each culture well, CPDye-labeled CD4+CD25+ T reg cells were incubated with irradiated (2,500 Rad) APCs at a 1:1 ratio and stimulated with 0.15 ?g/ml soluble anti-CD3 (2C11) and 500 U/ml recombinant IL-2 (PeproTech) in the presence or absence of recombinant IFN-? (PBL Biomedical Laboratories) for 72 h at 37°C, 5% CO2.
Tags: anti-mouse IL-2 S4B6-1 in cancer research; anti-mouse IL-2 S4B6-1 antibody in cancer research

Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2.
McKinstry, K. K., et al. Nat Commun. 2014 Nov 5;5:5377. doi: 10.1038/ncomms6377. PMID: 25369785
Autocrine IL-2 production by effectors, or high levels of exogenously administered IL-2, during this timeframe was required for the generation of almost all memory cells. ... IL-2 neutralization also reduced long-term survival of in vivo-generated polyclonal memory CD4 T cells responding to IAV. ... These data confirm that late IL-2 signals during the effector phase of the CD4 T-cell response are required for optimal memory generation. ... Strikingly, IL-2C treatment restored Il2 ?/? memory at 28 d.p.i. to WT levels but did not impact the peak expansion of Il2 ?/? cells at 7 d.p.i. ... Optimal rescue required a high dose of 2 ?g of IL-2, while 1 ?g led to reduced but still improved Il2 ?/? donor cell survival.
Tags: function of anti-mouse IL-2 S4B6-1; function of anti-mouse IL-2 S4B6-1 mAb

IL-2-dependent adaptive control of NK cell homeostasis.
Gasteiger, G., et al. J Exp Med. 2013 Jun 3;210(6):1179-87. doi: 10.1084/jem.20122571. PMID: 23650439
Thus, T reg cells restrain the IL-2?dependent CD4+ T cell help for CD127+ immature NK cells. ... T reg cells limited the IL-2?dependent homeostasis of these cells, and CD127+ NK cells accumulated in tumor-bearing and chronically infected mice. ... These observations suggested that CD25-dependent IL-2 signaling conferred a competitive advantage to immature CD127+ NK cells over their CD127? counterparts in the absence of T reg cell?mediated suppression and in tumor-bearing and chronically infected mice. ... Furthermore, blockade of IL-2 prevented the expansion of CD127+ NK cells in T reg cell?depleted mice. ... T reg cells control the homeostasis of these immature NK cells by restricting CD4+ T cell?dependent IL-2 availability.
Tags: anti-mouse IL-2 S4B6-1 antibody of low endotoxin; clone S4B6-1 of anti-mouse IL-2

For more references about Anti-mouse IL-2 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.