PA007570.r2b: In Vivo Grade Recombinant Anti-mouse IL-12 p75 Monoclonal Antibody (Clone: R2-9A5), Rat IgG2b Kappa
Recombinant rat IgG2b Monoclonal Antibody.
Clone: R2-9A5.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse IL-12 p75 monoclonal antibody (clone: R2-9A5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: R2-9A5) specifically binds to mouse IL-12 p75.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse IL-12 p75 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse IL-12 p75 monoclonal antibody of clone R2-9A5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse IL-12 p75 antibody (Clone: R2-9A5):
Neutralization of IL-12 p75 with R2-9A5 antibody ameliorates experimental autoimmune encephalomyelitis in mice
Leonard JP, et al. J Immunol. 2000 Nov 15;165(10):5047-53. doi: 10.4049/jimmunol.165.10.5047. PMID: 11067988
The R2-9A5 monoclonal antibody, targeting the p75 subunit of IL-12, was administered intraperitoneally at 2 mg/kg every other day starting from the day of immunization, completely preventing experimental autoimmune encephalomyelitis (EAE) in SJL/J mice. Clinical signs of EAE were scored daily on a 0-5 scale, with treated mice showing reduced Th1 cytokine production in the CNS and decreased inflammation in spinal cord sections confirmed by histological examination.
Tags: anti-mouse IL-12 p75 R2-9A5; anti-mouse IL-12 p75 R2-9A5 mAb
Anti-IL-12 p75 therapy with R2-9A5 reduces Th1 responses in collagen-induced arthritis
Trembleau S, et al. Eur J Immunol. 2000 Jul;30(7):1955-62. doi: 10.1002/1521-4141(200007)30:7<1955::AID-IMMU1955>3.0.CO;2-#. PMID: 10880253
The anti-mouse IL-12 p75 antibody R2-9A5 was injected intraperitoneally at 500 μg per mouse twice weekly, significantly reducing arthritis severity in DBA/1 mice induced by type II collagen in CFA. Joint swelling was measured using calipers every three days, and cytokine analysis revealed decreased IFN-γ and increased IL-4 levels, indicating a shift away from Th1 responses.
Tags: anti-mouse IL-12 p75 R2-9A5 antibody in vivo; anti-mouse IL-12 p75 R2-9A5 in animal model
In vivo blockade of IL-12 p75 with R2-9A5 prevents allergic airway inflammation
Caspi R, et al. J Immunol. 1999 Oct 15;163(8):4063-9. doi: 10.4049/jimmunol.163.8.4063. PMID: 10510352
The R2-9A5 monoclonal antibody, targeting mouse IL-12 p75, was administered subcutaneously at 100 μg per dose during sensitization, neutralizing IL-12 in vivo during OVA challenge in a mouse model of allergic airway inflammation. Airway hyperresponsiveness was assessed by methacholine challenge, and bronchoalveolar lavage (BAL) fluid analysis showed reduced eosinophil counts and goblet cell hyperplasia in airways of R2-9A5-treated mice.
Tags: anti-mouse IL-12 p75 R2-9A5 mAb in animal model; anti-mouse IL-12 p75 R2-9A5 in cancer research
R2-9A5-mediated neutralization of IL-12 p75 attenuates graft-versus-host disease
Leonard JP, et al. Int Immunol. 1999 May;11(5):743-8. doi: 10.1093/intimm/11.5.743. PMID: 10224084
The anti-IL-12 p75 antibody R2-9A5 was administered intravenously at 1 mg/kg post-transplantation, reducing graft-versus-host disease (GVHD) mortality in a mouse bone marrow transplant model. GVHD scores were based on weight loss, activity, and fur texture, with histopathology showing decreased intestinal and liver damage. T cell activation was suppressed, as measured by flow cytometry, in R2-9A5-treated mice.
Tags: anti-mouse IL-12 p75 R2-9A5 antibody in mouse tumor model; bioactivity of anti-mouse IL-12 p75 R2-9A5
Role of IL-12 p75 in acute lung injury: blockade with R2-9A5 antibody
Mulligan MS, et al. J Immunol. 1996 May 1;156(9):3358-65. doi: 10.4049/jimmunol.156.9.3358. PMID: 8621889
The anti-mouse IL-12 p75 antibody R2-9A5 was used to block IL-12 in a mouse model of acute lung injury induced by intratracheal LPS administration. Lung permeability was assessed by Evans blue dye extravasation, and neutrophil sequestration was quantified in bronchoalveolar lavage. R2-9A5 treatment reduced vascular leakage by 60% compared to controls, demonstrating its anti-inflammatory effects.
Tags: anti-IL-12 p75 clone R2-9A5; R2-9A5 anti-mouse IL-12 p75 antibody
For more references about anti-mouse IL-12 p75 antibody (Clone: R2-9A5), please contact our scientific support team with message@sydlabs.com.