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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant rat IgG2b Monoclonal Antibody.
Clone: R2-9A5.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse IL-12 p75 monoclonal antibody (clone: R2-9A5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: R2-9A5) specifically binds to mouse IL-12 p75.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse IL-12 p75 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse IL-12 p75 monoclonal antibody of clone R2-9A5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12.
Everts, B., et al. J Exp Med. 2016 Jan 11;213(1):35-51. doi: 10.1084/jem.20150235. PMID: 26712805
steady-state production of IL-12 by migratory CD103+ DCs, independent of signals from commensals or TLR-initiated events, was necessary and sufficient to exert the suppressive effects on Th2 response development. ... We identified constitutive production of IL-12 by Batf3-dependent migratory CD103+ DCs as the key mechanism through which these cells suppress type 2 immune responses. ... However, significant percentages of migratory CD103+ DCs continued to express YFP after infection. ... As a result, the majority of DCs expressing IL-12p40 in these LNs during both infections were of the migratory CD103+ type. ... We found that treatment of WT BALB/c mice with neutralizing anti?IL-12p70 antibody during H. polygyrus infection resulted in increased accumulation of RELM?+ p-Macs.
Tags: anti-mouse IL-12 p75 R2-9A5; anti-mouse IL-12 p75 R2-9A5 mAb
Fimbriated Salmonella enterica serovar typhimurium abates initial inflammatory responses by macrophages.
Pascual, D. W., et al. Infect Immun. 2002 Aug;70(8):4273-81. doi: 10.1128/IAI.70.8.4273-4281.2002. PMID: 12117936
Only minute levels of IL-12 p70 were induced by Salmonella vector-infected macrophages, and none was induced by Salmonella-CFA/I-infected macrophages. ... The absence of IL-12 was not due to overt increases in production of either IL-12 p40 or IL-10. ... Infection of RAW 264.7 cells or peritoneal macrophages with either Salmonella construct showed no difference in macrophage viabilities. ... This evidence shows that the expression of CFA/I fimbriae alters how macrophages recognize or process salmonellae and prevents the rapid onset of proinflammatory cytokines which is typical during Salmonella infections. ... Only minute levels of IL-12 p70 were induced by Salmonella vector-infected macrophages, and none was induced by Salmonella-CFA/I-infected macrophages.
Tags: anti-IL-12 p75 clone R2-9A5; R2-9A5 anti-mouse IL-12 p75 antibody
For more references about Anti-mouse IL-12 p75 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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