Anti-Mouse IL-4 Monoclonal Antibodies

PA007534.r1: In Vivo Grade Recombinant Anti-mouse IL-4 Monoclonal Antibody (Clone: 11B11), Rat IgG1 Kappa

Recombinant rat IgG1 Monoclonal Antibody.
Clone: 11B11.
Isotype: Rat IgG1 kappa.
Source: The anti-mouse IL-4 monoclonal antibody (clone: 11B11) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 11B11) specifically binds to mouse IL-4.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse IL-4 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse IL-4 monoclonal antibody of clone 11B11 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse IL-4 Monoclonal Antibody:

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.
Gaya, M., et al. Cell. 2018 Jan 25;172(3):517-533.e20. doi: 10.1016/j.cell.2017.11.036. PMID: 29249358
These processes are strictly dependent on co-stimulatory signals, mainly CD40-L, ICOS, and cytokines, such as interferon ? (IFN-?), interleukin-4 (IL-4), and IL-21, provided by follicular helper T (TfH) cells. ...Instead, NKT cells promote B cell immunity through the early and localized production of IL-4 at the follicular borders, a process that is likely driven by CXCR3 and requires initial priming by resident macrophages through CD1d and IL-18. ...Therefore, our results indicate that the production of IL-4 by NKT cells immediately after viral infection contributes to a pre-TfH IL-4 wave at the follicular borders that facilitates the initial seeding of germinal center cells. ...Interestingly, influenza infection not only results in an upregulation of activation markers but also in a robust production of IFN-? and IL-4, with a similar percentage of NKT cells producing either cytokine. ...These observations demonstrate that, although the population of NKT cells is much smaller than those of conventional T cells, they represent the main source of IL-4 in lymph nodes at the early stages of influenza infection.
Tags: anti-mouse IL-4 11B11; anti-mouse IL-4 (11B11) in vivo antibody

Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.
Clever, D., et al. Cell. 2016 Aug 25;166(5):1117-1131.e14. doi: 10.1016/j.cell.2016.07.032. PMID: 27565342
Naive CD4+ T cells were activated with plate-bound anti-CD3 and soluble anti-CD28 (5??g?ml?1 each; eBioscience) in media for 72h either under: Th0 conditions (media alone); Th1 conditions (IL-12 (10?ng?ml?1, R&D Systems), anti-IL-4 neutralizing antibodies (10??g?ml?1); Th17 conditions (IL-6 (20?ng?ml?1, R&D Systems), human TGF-?1 (0.2?ng?ml?1, R&D Systems), anti-IFN-? neutralizing antibodies (10??g?ml?1) and anti-IL-4 neutralizing antibodies (10??g?ml?1); or iTreg conditions (human TGF-?1 (0-2?ng?ml?1, as indicated)). ...The following fluorescent dye-conjugated antibodies against surface and intracellular antigens were used: anti-FOXP3 (FJK-16 s), anti-IL-17A (eBio17B7), anti-IFN-? (XMG1.2), anti-Tbet (eBio4B10), anti-Ror gamma (t) (B2D), anti-CD304 (Nrp-1, 3DS304M), anti-mouse TCR-beta (H57-597), anti-IL-13 (eBio13A), anti-Granzyme B (NGZB), and anti-Perforin (eBioOMAK-D) (eBioscience); anti-CD45.1 (A20), anti-CD4 (RM4-5), anti-CD25 (PC61), anti-CD62L (MEL-14), anti-CD44 (IM7), anti-CD8a (53-6.7), anti-CD152 (CTLA4, UC10-4F10-11), anti-GITR (DTA-1), anti-IL-4 (11B11), anti-IL-5 (TRFK5) (BD Biosciences); Anti-mouse IgG Fab2 (Cell Signaling); Anti-mouse HIF1? (Abcam). ...All RNA-Seq analyses were performed using ? 2 biological replicates. Raw data from replicate measurements are publically available from the GEO repository. ...Paired-end RNA sequencing was performed on a HiSeq 2000 (Illumina). ...For measurement of antinuclear (ANA) and anti-dsDNA autoantibodies, ELISA assays were performed on mouse serum according to manufacturer’s instructions (Alpha Diagnostic International).
Tags: anti-mouse IL-4 11B11 antibody; anti-mouse IL-4 antibody (clone 11B11)

The protease cathepsin L regulates Th17 cell differentiation.
Hou, L., et al. J Autoimmun. 2015 Dec;65:56-63. doi: 10.1016/j.jaut.2015.08.006. PMID: 26343333
IL-17 producing T helper cells (Th17 cells) are critically important for protective immunity of mucosal surfaces against fungal infections and extracellular bacteria, but are also implicated in the pathology of severe inflammatory and autoimmune diseases including multiple sclerosis, psoriasis, rheumatoid arthritis, and inflammatory bowel disease. ...To evaluate the effect of SerpinB1 on Th17 differentiation, we immunized mice with keyhole limpet hemocyanin (KLH) and evaluated antigen recall responses seven days later. ...The overall findings strongly suggest that catL, serpinB1 and AEP form an intracellular regulatory module that contributes to the control of Th17 differentiation. ...We thank Guo-Ping Shi for providing reagents and advice, Thomas Reinheckel and Johanna Joyce for providing ctsh?/? mice, Dieter Bromme for cathepsin V, Phillip Bird for initial discussions and material and Norma Gerard for use of CFX96 Real-Time System. ...Overall, the dynamic regulatory program that coordinates Th17 differentiation involves sequential developmental stages coordinated by a network of highly interactive antagonistic interactions of Th17 promoting- and a smaller number of Th17 suppressing-modules.
Tags: anti-mouse IL-4 11B11 in animal model; anti-mouse IL-4 antibody (clone 11B11)

Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells.
Kim, Y. U., et al. PLoS One. 2015 Mar 13;10(3):e0120294. doi: 10.1371/journal.pone.0120294. PMID: 25768299
Rocky Hill, NJ), 40 ng/ml recombinant mouse IL-23 (R&D systems), 10 ?g/ml anti-mouse IFN-? (clone XMG 1.2, BioXcell) and 10 ?g/ml anti-mouse IL-4 (clone 11B11, BioXcell). ...For cytokine blockade, 10 ?g/ml of recombinant human Fc-G1 (Human Fc-G1, BioXCell), anti-IL-17A (clone 50104, R&D Systems) or recombinant mouse IL-21R-Fc Chimera (R&D Systems) was added into the B and T cell co-culture every other day. ...To measure the levels of antibodies to dsDNA and histone in circulation, serum was collected from the wild type or BXD2 mice at various time points and was measured by ELISA as described previously. ...Sera were diluted in PBS with 1% of FBS, were transferred to the plates and were incubated for 2 hours at room temperature. ...Total RNA was extracted from splenocytes (1×106 cells) or in vitro co-cultured cells with TRIzol (Invitrogen) and reverse transcribed using amfiRivert reverse transcriptase (GenDepot, Baker, TX) according to the manufacturer’s protocol.
Tags: anti-mouse IL-4 11B11 in cancer research; anti-mouse IL-4 antibody 11B11

Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter.
Hill, E. V., et al. Eur J Immunol. 2015 Apr;45(4):1103-15. doi: 10.1002/eji.201444661. PMID: 25627813
There was an increase in polarized Th2 cells producing IL-10 in response to GSK3 inhibition (Fig. 1D) and there was a particularly large increase in cells expressing both IL-10 and IL-4. ...IL-2 was added on day 3 of culture and intracellular cytokine staining for IL-4 and IL-10 carried out on day 7. ...Data are plots gated on live CD4+ cells and are representative of four independent experiments. (E) Pooled quantitation of IL-10+/ IL-4+ cells from four independent experiments as shown in (D). ...There was a small increase in IL-10 production in Th1 and Th17 polarizing cells and a larger increase in IL-10 in Th2 polarizing cells; interestingly this was mainly from non-IL-4-expressing cells, unlike established polarized Th2 cells where the increase was predominantly in IL-4+IL-10+ cells. ...This beneficial effect of a rise in IL-10 in the presence of IL-4, as seen in this study, suggests that GSK3 inhibition may be a useful tool in allergen-specific immunotherapy.
Tags: anti-mouse IL-4 11B11 in mouse tumor model; clone 11B11 of mouse IL-4 antibody

A critical role for transcription factor Smad4 in T cell function that is independent of transforming growth factor ? receptor signaling.
Gu, A. D., et al. Immunity. 2015 Jan 20;42(1):68-79. doi: 10.1016/j.immuni.2014.12.019. PMID: 25577439
Fluorescence-conjugated anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-CD5 (53-7.3), anti-CD24 (30-F1), anti-CD25 (PC61.5), anti-CD44 (IM7), anti-CD69 (H1.2F3), anti-CD62L (MEL-14), anti-CD45.1 (A20), anti-CD45.2 (104), anti-IFN-? (XMG1.2), anti-IL-4 (11B11), anti-IL-2 (JES6-5H4), and anti-Foxp3 (FJK-16 s) (eBioscience), Myc (9E10, Santa Cruz), as well as Annexin V and 7-amino-actinomycin D (BD Biosciences) were used. ...Th1 cells were differentiated in the presence of 20 ng/ml rIL-12 (R&D Systems) and 20 ?g/ml anti-IL-4 (11B11, BioXcell). ...Th2 cells were differentiated in the presence of 20 ng/ml rIL-4 (R&D Systems) and 20 ?g/ml anti-IFN-? (XMG1.2, BioXcell). ...Bone marrow cells were isolated from the femur bones of sex- and age-matched Cd4-cre-Tgfbr2fl/fl (CD45.2+) or Cd4-cre-Tgfbr2fl/flSmad4fl/fl (CD45.2+) mice and wild-type (CD45.1+) mice. ...The ChIP assay was done according to the protocol of Upstate Biotechnology.
Tags: anti-mouse IL-4 11B11; anti-mouse IL-4 11B11 mAb

The oncoprotein and transcriptional regulator Bcl-3 governs plasticity and pathogenicity of autoimmune T cells.
Tang, W., et al. Immunity. 2014 Oct 16;41(4):555-66. doi: 10.1016/j.immuni.2014.09.017. PMID: 25367572
Naive CD4+ T cells were isolated from 8-week-old healthy mice with the Miltenyi Naive CD4+ T cell Isolation Kit II and seeded at a concentration of 1 × 105 cells per well in 96-well plates coated with 1 ?g/ml anti-CD3 (145-2C11). Anti-CD28 (37.51) was added to the media at 2 ?g/ml and for Th1 cell differentiation also 10 ng/ml IL-12 and 10 ?g/ml anti-IL-4 (11B11); for Treg cells, 100 U/ml IL-2, 2 ng/ml TGF-?, 10 ?g/ml anti-IL-12 (C18.2), 10 ?g/ml anti-IFN-? (XMG1.2), and 10 ?g/ml anti-IL-4; for Th17 cells, 20 ng/ml IL-6, 5 ng/ml TGF-?, 10 ?g/ml anti-IL-12 (C18.2), 10 ?g/ml anti-IFN-? (XMG1.2), and 10 ?g/ml anti-IL-4; for Th17+ cells, in addition, IL-1 (10 ng/ml), IL-21 (50 ng/ml), and IL-23 (20 ng/ml). ...All cytokines and antibodies noted above were purchased from PeproTech and BioXCell, respectively, except anti-IL-12 (C18.2) (eBioscience). ...In some experiments, Rag1?/? mice were treated for 4 weeks with ampicillin (1 g/l), vancomycin (500 mg/l), neomycin sulfate (1 g/l), and metronidazole (1 g/l) in drinking water for 4 weeks prior to Th1 cell transfers. Also, in some transfers, recipients were injected i.p. with 0.2 mg of anti-IL23 p19 (BD Biosciences) or anti-IL12/IL23 p40 (BioXCell) neutralizing antibodies/mouse every 3 days. ...RNA was isolated using the RNeasy (QIAGEN) according to the manufacturer’s instructions. cDNA was synthesized with Superscript III (Invitrogen). ...Gene expression was quantified with the TaqMan real-time PCR primers (Applied Biosystems). The results were normalized against ?-actin.
Tags: anti-mouse IL-4 11B11 in vivo; anti-mouse IL-4 11B11 in animal model

Sequential transcriptional changes dictate safe and effective antigen-specific immunotherapy.
Burton, B. R., et al. Nat Commun. 2014 Sep 3;5:4741. doi: 10.1038/ncomms5741. PMID: 25182274
Briefly, Tg4 splenocytes were cultured for 10 days with 10??g?ml?1 MBP Ac1-9[4K], 10?ng?ml?1 IL-1?, 2?ng?ml?1 transforming growth factor-?, 25?ng?ml?1 IL-6 (all cytokines from PeproTech), 10??g?ml?1 anti-IL-4 (11b11), 50??g?ml?1 anti-IFN-? (XMG1.2) (both from BioXcell), supplemented with 10?ng?ml?1 rmIL-23 on day 2 (eBioscience). ...For in vitro intracellular dye dilution proliferation assays, splenic CD4+ T cells were isolated from untreated Tg4 mice using a CD4+ T-cell isolation kit (Miltenyi Biotec), and labelled with 0.5??M CFSE (Invitrogen). ...CD4+ T cells were isolated using a CD4+ T-cell isolation kit (Miltenyi Biotec) from Tg4 Rag-1-deficient mice at six sequential stages of EDI and from PBS-treated controls, 2?h after s.c. challenge with 80??g of MBP Ac1-9[4Y] (three mice per group). ...Reverse transcription and amplification was performed at the University of Bristol Genomics Facility using the Ambion WT Expression kit (Applied Biosystems), fragmentation and labelling using GeneChip WT Terminal Labelling and Hybridization kit before hybridization to GeneChip Mouse Gene 1.0 ST arrays (both Affymetrix). ...Briefly, the gene expression matrix was first trained by SOM with Gaussian kernel.
Tags: anti-mouse IL-4 11B11 mAb in animal model; function of anti-mouse IL-4 11B11

IL-27 and IL-12 oppose pro-inflammatory IL-23 in CD4+ T cells by inducing Blimp1.
Heinemann, C., et al. Nat Commun. 2014 May 6;5:3770. doi: 10.1038/ncomms4770. PMID: 24796719
While the cytokine phenotypes of IFN-?-producing Th1 cells and IL-4-producing Th2 cells are relatively stable4, Th17 cells appear to be more plastic. ...CD4+ T cells from control or Blimp1 CKO mice were differentiated in vitro into Th1 cells (IL-12 plus anti-IL-4), Th2 cells (IL-4 plus anti-IL-12) and Th17 cells (TGF-? plus IL-6) and assessed for intracellular cytokines on day 4. Frequencies of IL-10+ producing T cells are depicted. ...If not otherwise indicated, Th1 cells were differentiated in the presence of 10?ng?ml?1 IL-12 and 10??g?ml?1 anti-IL-4 (11B11, BioXcell), Th17 cells were differentiated in the presence of 0.5?ng?ml?1 TGF-?1 and 50?ng?ml?1 IL-6 and Tr-1 cells were differentiated with 0.5?ng?ml?1 TGF-?1 and 100?ng?ml?1 IL-27. ...For ICCS, cells were isolated and stimulated in culture medium containing 50?ng?ml?1 PMA, 1??g?ml?1 ionomycin (both Sigma-Aldrich) and 1??l?ml?1 GolgiStop (monensin, BD Biosciences) at 37?°C and 5% CO2 for 3?h. ...All antibodies were used 1:100, except for IFN-? (1:200). For Foxp3 detection, the Foxp3 staining kit from eBioscience was used according to the manufacturer’s recommendation.
Tags: anti-mouse IL-4 11B11; anti-IL-4 clone 11B11

Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2.
McKinstry, K. K., et al. Nat Commun. 2014 Nov 5;5:5377. doi: 10.1038/ncomms6377. PMID: 25369785
In brief, naive WT or Il2?/? CD4 T cells were cultured with an equal number of irradiated APC (2 × 105 per ml) in the presence of exogenous IL-2 (20?ng?ml?1), 2?ng?ml?1 IL-12 (Peprotech), 10??g?ml?1 anti-IL-4 Ab (11B11; Bioxcell) and 5??M OVAII peptide. ...In some experiments, total polyclonal CD4 T cells were isolated from IAV-primed C57BL/6.Thy1.1 mice by positive selection with CD4 MACS beads (Miltenyi), and CFSE labelled as previously described. ...Influenza A/PR8-OVAII (H1N1) and A/HKx31-OVAII (H3N2) viruses were produced in the allantoic cavity of embryonated hen eggs from stock obtained from P. ...Donor cell injection and viral infection occurred on the same day. ...Pulmonary viral titre was determined by quantitation of viral RNA.
Tags: bioactivity of anti-mouse IL-4 11B11; bioactivity of anti-mouse IL-4 11B11 mAb

Prevention of type 1 diabetes through infection with an intestinal nematode parasite requires IL-10 in the absence of a Th2-type response.
Mishra, P. K., et al. Mucosal Immunol. 2013 Mar;6(2):297-308. doi: 10.1038/mi.2012.71. PMID: 22806101
Most recently, infection of NOD-IL-4?/? mice with Litosomoides sigmodontis remarkably showed that the development of diabetes is still prevented despite blockade of the Th2-type response. ...The immune response to Hp is characterized by a potent polarized Th2-type response with pronounced elevations in IL-4 and IL-13. ...These studies indicate that Hp infection can control development of T1D through independent mechanisms involving either IL-4 or IL-10. ...Histological analysis of ?-cell infiltration was performed with Hp-inoculated NOD-IL-4?/? mice and untreated NOD-IL-4?/? mice at 13 weeks of age, a time point when insulitis is readily detectable but has not yet resulted in total ?-cell destruction. ...As shown in Figure 1c and d, Hp inoculation of NOD-IL-4?/? mice at 5 weeks of age markedly reduced both invasive insulitis and more mild peri-insulitis.
Tags: anti-mouse IL-4 11B11 in cancer research; anti-mouse IL-4 11B11 in mouse tumor model

Divergent requirement for G?s and cAMP in the differentiation and inflammatory profile of distinct mouse Th subsets.
Li, X., et al. J Clin Invest. 2012 Mar;122(3):963-73. doi: 10.1172/JCI59097. PMID: 22326954
Furthermore, WT CD4+ T cells had an 81-fold increase in IFN-??producing cells (Th1, Figure 4B) but a similar number of IL-4?producing cells (Th2, Figure 4C) compared with Gnas?CD4 CD4+ T cells. ...The CD4+ T cells were then stimulated with PMA/ionomycin for 4 hours. IL-17A, IFN-?, IL-4, and Foxp3 levels were determined by flow cytometry. ...8Br-cAMP restored the expression of Gnas?CD4 IL-17+ and IFN-?+ but did not alter IL-4+ or Foxp3+ CD4+ T cells, implying that reduced cAMP accumulation in Gnas?CD4 CD4+ T cells selectively modulates Th17 and Th1 differentiation. ...Naive CD4+ T cells (1 × 106/ml) were cultured in medium with soluble anti-CD3 Ab (0.25 ?g/ml), anti-CD28 Ab (1 ?g/ml), and neutralizing antibodies for IL-4 (10 ?g/ml) and IFN-? (10 ?g/ml). ...Naive CD4+ T cells were cultured in goat anti-hamster Ab?coated culture plates in complete IMDM medium with soluble anti-CD3 Ab (5 ?g/ml) and anti-CD28 Ab (1 ?g/ml), added with IL-12 (20 ng/ml) and neutralizing anti?IL-4 Ab (10 ?g/ml) for Th1 differentiation; or IL-4 (10 ng/ml) and neutralizing anti?IFN-? Ab (10 ?g/ml) for Th2 differentiation.
Tags: function of anti-mouse IL-4 11B11 antibody; bioactivity of anti-mouse IL-4 11B11

Fyn promotes Th17 differentiation by regulating the kinetics of ROR?t and Foxp3 expression.
Ueda, A., et al. J Immunol. 2012 Jun 1;188(11):5247-56. doi: 10.4049/jimmunol.1102241. PMID: 22539787
Th17 cells constitute a proinflammatory CD4+ T cell subset that is important for microbial clearance, but also are implicated as propagators of various autoimmune pathologies. ...Evidence suggests that Th17 cells share common progenitors with immunosuppressive CD4+ inducible regulatory T cells (TREG) and that the developmental pathways of these two subsets are reciprocally regulated. ...The defect in IL-17 expression occurred independently of the ectopic Foxp3 expression and correlated with a delay in retinoic acid-related orphan receptor ?t upregulation and an inability to maintain normal STAT3 activation. ...Fyn-deficient Th17 cells also exhibited delayed upregulation of Il23r, Il21, Rora, and Irf4, as well as aberrant expression of Socs3, suggesting that Fyn may function upstream of a variety of molecular pathways that contribute to Th17 polarization. The fyn?/? mice had fewer IL-17+CD4+ T cells in the large intestinal lamina propria compared with littermate controls. ...Furthermore, after transfer of either wild-type or fyn?/? naive CD4+ T cells into Rag1?/? hosts, recipients receiving fyn?/? cells had fewer IL-17?producing T cells, indicating that Fyn may also regulate Th17 differentiation in vivo.
Tags: anti-mouse IL-4 antibody (11B11); anti-IL-4 clone 11B11

For more references about Anti-mouse IL-4 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.