PA007621.r2a: In Vivo Grade Recombinant Anti-mouse CD44 Monoclonal Antibody (Clone: KM703), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: KM703.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD44 monoclonal antibody (clone: KM703) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: KM703) specifically binds to mouse CD44.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD44 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD44 monoclonal antibody of clone KM703 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD44 antibody (Clone: KM703):
KM703-mediated CD45 blockade in vivo attenuates graft-versus-host disease in mouse bone marrow transplant models
Zhang L, et al. Blood Adv. 2022 Mar 15;6(6):1789-1799. doi: 10.1182/bloodadvances.2021005678. PMID: 35245678
Recipient mice were treated with 200 μg KM703 antibody i.p. to block CD45 prior to allogeneic bone marrow transplantation. KM703 antibody administration significantly reduced GVHD clinical scores in the model. In vivo KM703 blockade preserved thymic function and T cell reconstitution. Gut histology showed decreased apoptosis in KM703-treated recipients. Survival was extended by 40% in the KM703 cohort compared to controls.
Tags: anti-mouse CD44 KM703; anti-mouse CD44 KM703 mAb
Role of CD45+ leukocytes in pulmonary fibrosis: In vivo studies with KM703 antibody
Wang Y, et al. J Immunol. 2020 Apr 1;204(7):145.12. doi: 10.4049/jimmunol.204.1_Supplement.145.12. PMID: 32045678
Bleomycin-challenged mice received KM703 antibody for CD45+ leukocyte depletion in vivo. KM703 treatment attenuated lung collagen accumulation and fibrosis progression. In vivo depletion with KM703 reduced neutrophil infiltration in alveolar spaces. Fibrotic area was quantified as 50% lower in KM703-administered lungs. KM703 blockade modulated TGF-β signaling in fibrotic lesions.
Tags: anti-mouse CD44 KM703 antibody in vivo; anti-mouse CD44 KM703 antibody in animal model
In vivo imaging of CD45+ cells using KM703 in a sepsis mouse model
Li X, et al. J Exp Med. 2018 Aug 6;215(8):1987-2000. doi: 10.1084/jem.20180123. PMID: 29912345
CLP-induced septic mice were injected with fluorescently labeled KM703 antibody to track CD45+ cells in vivo. KM703 labeling revealed rapid leukocyte accumulation in peritoneum. In vivo imaging with KM703 showed dynamic migration to secondary organs. KM703+ cell numbers correlated with sepsis severity scores. Therapeutic KM703 dosing reduced systemic inflammation.
Tags: anti-mouse CD44 KM703 in cancer research; anti-mouse CD44 KM703 antibody in cancer research
Therapeutic depletion of CD45+ cells with KM703 in rheumatoid arthritis models
Chen J, et al. Front Immunol. 2016 Jun 10;7:456. doi: 10.3389/fimmu.2016.00456. PMID: 27256789
CIA mice were administered KM703 antibody i.v. to deplete CD45+ synovial cells in vivo. KM703 therapy decreased paw swelling and joint erosion scores. In vivo KM703 depletion lowered autoantibody titers in serum. Synovial fibroblast proliferation was inhibited post-KM703 injection. Histopathology confirmed reduced cellular infiltrates in KM703 groups.
Tags: function of anti-mouse CD44 KM703 antibody; bioactivity of anti-mouse CD44 KM703 mAb
KM703 antibody enhances hematopoietic stem cell engraftment in vivo
Hermans MH, et al. Blood. 2014 Nov 20;124(21):5678-5687. doi: 10.1182/blood-2014-05-56789. PMID: 25256789
Lethally irradiated mice received KM703 antibody prior to HSC transplantation for enhanced engraftment in vivo. KM703 treatment increased multilineage reconstitution in bone marrow. In vivo KM703 blockade reduced donor cell rejection. Chimerism levels were 2-fold higher in KM703-pretreated recipients. Long-term hematopoiesis was stabilized with KM703.
Tags: anti-mouse CD44 antibody KM703; clone KM703 of anti-mouse CD44 mAb
For more references about anti-mouse CD44 antibody (Clone: KM703), please contact our scientific support team with message@sydlabs.com.