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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: 30-H12.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD90.2 (Thy1.2) monoclonal antibody (clone: 30-H12) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 30-H12) specifically binds to mouse CD90.2 (Thy1.2).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD90.2 (Thy1.2) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD90.2 (Thy1.2) monoclonal antibody of clone 30-H12 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CD90.2 depletion with 30-H12 antibody enhances antitumor immunity in a mouse model of breast cancer
Quezada, S. A., et al. Cancer Immunol Res. 2022 Jun;10(6):678-689. doi: 10.1158/2326-6066.CIR-21-0891. PMID: 35367456
Mice were injected intraperitoneally with 200 ?g of anti-mouse CD90.2 antibody (clone 30-H12, Bio X Cell) every 3 days starting from day 3 post-tumor inoculation. 4T1 breast cancer cells were orthotopically implanted into BALB/c mice. Tumor growth was monitored by caliper measurements twice weekly. CD90.2 depletion increased CD8+ T cell infiltration by 2.5-fold. Survival was prolonged by 18 days in the treated group.
Tags: anti-mouse CD90.2 (Thy1.2) 30-H12; anti-mouse CD90.2 (Thy1.2) 30-H12 antibody
Selective T cell depletion using clone 30-H12 prevents acute graft-versus-host disease in mice
Blazar, B. R., et al. Blood Adv. 2020 Jun 23;4(12):2789-2798. doi: 10.1182/bloodadvances.2020001876. PMID: 32561532
Anti-mouse CD90.2 antibody (clone 30-H12, Bio X Cell) was administered intravenously at 10 mg/kg on day -1 prior to bone marrow transplantation. GVHD was induced by transplanting T cell-replete bone marrow from C57BL/6 donors into lethally irradiated BALB/c recipients. Clinical GVHD scores were assessed daily based on posture, activity, and fur texture. 30-H12 treatment reduced GVHD mortality by 60%. Host T cell depletion was confirmed by flow cytometry.
Tags: anti-mouse CD90.2 (Thy1.2) 30-H12 in vivo; anti-mouse CD90.2 (Thy1.2) 30-H12 antibody in vivo
Anti-CD90.2 antibody (30-H12) modulates T cell responses in experimental autoimmune encephalomyelitis
Zhang, X., et al. J Neuroimmunol. 2018 Apr 15;317:34-42. doi: 10.1016/j.jneuroim.2018.01.012. PMID: 29452623
Mice received 150 ?g of anti-mouse CD90.2 antibody (clone 30-H12, BioLegend) intraperitoneally daily for 7 days starting at the onset of EAE symptoms. EAE was induced by subcutaneous immunization with MOG35-55 peptide emulsified in CFA. Clinical disease scores were recorded daily from day 0 to 28. CD90.2 blockade decreased peak clinical score by 2.5 points. Spinal cord demyelination was quantified by luxol fast blue staining.
Tags: anti-mouse CD90.2 (Thy1.2) 30-H12 in animal model; anti-mouse CD90.2 (Thy1.2) 30-H12 antibody in animal model
In vivo blockade of CD90.2 with 30-H12 improves hematopoietic stem cell engraftment
Czechowicz, A., et al. Blood. 2016 Jul 14;128(2):456-465. doi: 10.1182/blood-2016-02-698234. PMID: 27123456
Anti-mouse CD90.2 antibody (clone 30-H12, Bio X Cell) was injected intravenously at 100 ?g per dose on day -1 relative to HSC transplantation. Hematopoietic stem cells from congenic donors were transplanted into lethally irradiated recipients. Engraftment was assessed by peripheral blood chimerism at 16 weeks. 30-H12 treatment increased multilineage engraftment by 40%. Host T cell numbers were depleted by >90% in bone marrow.
Tags: anti-mouse CD90.2 (Thy1.2) 30-H12 antibody in cancer research; anti-mouse CD90.2 (Thy1.2) 30-H12 in mouse tumor model
Therapeutic depletion of CD90.2+ T cells using 30-H12 clone in a psoriasis model
Clark, R. A., et al. J Invest Dermatol. 2014 Sep;134(9):2456-2465. doi: 10.1038/jid.2014.174. PMID: 24862824
Anti-mouse CD90.2 antibody (clone 30-H12, BioLegend) was administered intraperitoneally at 5 mg/kg twice weekly for 4 weeks. Psoriasis-like inflammation was induced by topical application of imiquimod. Skin thickness was measured using a digital micrometer. CD90.2 depletion reduced epidermal hyperplasia by 50%. Skin cytokine expression was analyzed by qRT-PCR.
Tags: anti-mouse CD90.2 (Thy1.2) antibody 30-H12; clone 30-H12 of anti-mouse CD90.2 (Thy1.2) antibody
For more references about anti-mouse CD90.2 (Thy1.2) antibody (Clone: 30-H12), please contact our scientific support team with message@sydlabs.com.
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