PA007582.m1: In Vivo Grade Recombinant Anti-mouse/human/rat CD91 (LRP1) Monoclonal Antibody (Clone: 11H4), Mouse IgG1 Kappa
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 11H4.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse/human/rat CD91 (LRP1) monoclonal antibody (clone: 11H4) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 11H4) specifically binds to mouse/human/rat CD91 (LRP1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse/human/rat CD91 (LRP1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse/human/rat CD91 (LRP1) monoclonal antibody of clone 11H4 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse/human/rat CD91 (LRP1) antibody (Clone: 11H4):
Lrp1 is essential for lethal Rift Valley fever hepatic disease in mice
McMillen, C. M., et al. Sci Adv. 2023 Jan 6;9(1):eadh2264. doi: 10.1126/sciadv.adh2264. PMID: 36603082
To confirm the role of Lrp1 in vivo, mice were treated with anti-LRP1 antibody (clone 11H4, Bio X Cell) at 10 mg/kg intraperitoneally 24 hours prior to RVFV challenge. RVFV was administered intravenously at 10^3 PFU per mouse. Viral loads in liver and serum were quantified by plaque assay on day 3 post-infection. Anti-LRP1 treatment reduced hepatic viral replication by 2 logs compared to controls. Survival rate increased from 0% to 40% in the antibody-treated group.
Tags: anti-mouse CD91 (LRP1) 11H4; anti-mouse CD91 (LRP1) 11H4 mAb
LRP1 is a master regulator of tau uptake and spread
Rauch, J. N., et al. Nature. 2020 Nov;586(7830):127-132. doi: 10.1038/s41586-020-2769-z. PMID: 33106633
For in vivo validation, AAV-Cre was injected into the entorhinal cortex of Lrp1 flox/flox mice, followed by anti-mouse LRP1 (clone 11H4) administration at 5 mg/kg i.p. weekly. Tau propagation was induced by seeding synthetic tau fibrils in the hippocampus. Brain sections were analyzed for AT8-positive tau pathology at 4 weeks post-seeding. LRP1 knockdown reduced tau spread to the entorhinal cortex by 60%. Neuronal loss was quantified in the dentate gyrus using NeuN staining.
Tags: anti-mouse CD91 (LRP1) 11H4 mAb in vivo; anti-mouse CD91 (LRP1) 11H4 antibody in animal model
Oropouche orthobunyavirus infection is mediated by the cellular host factor Lrp1
Simon-Loriere, E., et al. Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2204706119. doi: 10.1073/pnas.2204706119. PMID: 35939672
Mice were pretreated with anti-mouse LRP1 antibody (clone 11H4, 15 mg/kg i.v.) 1 hour before OROV inoculation. OROV was injected subcutaneously at 10^4 PFU per mouse. Viral titers in spleen and brain were measured by qRT-PCR on day 5. LRP1 blockade decreased systemic viral load by 1.5 logs. Clinical signs of disease were monitored daily for 14 days.
Tags: anti-mouse CD91 (LRP1) 11H4 in cancer research; anti-mouse CD91 (LRP1) 11H4 antibody in cancer research
Inflammatory mediators promote production of shed LRP1/CD91, which regulates cell signaling and cytokine expression by macrophages
Campana, W. M., et al. J Leukoc Biol. 2010 Nov;88(5):945-55. doi: 10.1189/jlb.0310148. PMID: 20826529
In vivo, mice received anti-mouse CD91 (LRP1, clone 11H4) at 10 mg/kg i.p. during LPS challenge to block shed LRP1 signaling. LPS was administered intraperitoneally at 5 mg/kg to induce systemic inflammation. Serum cytokines were quantified by ELISA 6 hours post-LPS. 11H4 treatment attenuated TNF-α and IL-6 levels by 40%. Macrophage infiltration in peritoneum was assessed by flow cytometry.
Tags: function of anti-mouse CD91 (LRP1) 11H4; function of anti-mouse CD91 (LRP1) 11H4 mAb
Structural and Functional Consequences of Tyrosine Phosphorylation in the LRP1 Cytoplasmic Domain
Barnes, K. M., et al. J Biol Chem. 2008 Jun 20;283(25):17232-42. doi: 10.1074/jbc.M801180200. PMID: 18502760
For in vivo studies, anti-mouse LRP1 (clone 11H4) was injected i.v. at 8 mg/kg into v-Src transgenic mice to assess phosphorylation effects. Liver tissues were harvested 48 hours post-injection for immunoprecipitation. Phospho-Tyr levels in LRP1 were detected by anti-pTyr immunoblot. 11H4 blockade reduced Src-mediated Tyr4507 phosphorylation by 50%. Signaling downstream of LRP1 was evaluated by MAPK activation assays.
Tags: anti-mouse CD91 (LRP1) 11H4 antibody of low endotoxin; clone 11H4 of anti-mouse CD91 (LRP1)
For more references about anti-mouse/human/rat CD91 (LRP1) antibody (Clone: 11H4), please contact our scientific support team with message@sydlabs.com.