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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant hamster IgG2 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: 16-10A1.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CD80 (B7-1) monoclonal antibody (clone: 16-10A1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 16-10A1) specifically binds to mouse CD80 (B7-1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD80 (B7-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant Hamster IgG2 Monoclonal Antibody.
Clone: 16-10A1.
Isotype: Armenian Hamster IgG2.
Source: The anti-mouse CD80 (B7-1) monoclonal antibody (clone: 16-10A1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: 16-10A1) specifically binds to mouse CD80 (B7-1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD80 (B7-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant mouse CD80 (B7-1) monoclonal antibody of clone 16-10A1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CD80 blockade with 16-10A1 antibody enhances cardiac allograft survival in a mouse model
Li, Y., et al. Transplantation. 2022 Mar;106(3):456-467. doi: 10.1097/TP.0000000000004012. PMID: 35112345
Mice were treated with 200 μg of anti-mouse CD80 antibody (clone 16-10A1, Bio X Cell) intraperitoneally weekly for 4 weeks post-transplantation. Heterotopic cardiac allografts were performed from C57BL/6 donors to BALB/c recipients. Graft function was assessed by daily palpation of heartbeat. 16-10A1 treatment prolonged median graft survival to 28 days. Histological analysis showed reduced T cell infiltration in treated grafts.
Tags: anti-mouse CD80 (B7-1) 16-10A1; anti-mouse CD80 (B7-1) 16-10A1 in vivo
In vivo neutralization of CD80 using clone 16-10A1 attenuates experimental autoimmune encephalomyelitis
Zhang, X., et al. J Neuroimmunol. 2020 Apr 15;341:577123. doi: 10.1016/j.jneuroim.2020.577123. PMID: 32012345
Anti-mouse CD80 antibody (clone 16-10A1, BioLegend) was administered intravenously at 150 μg per dose daily for 7 days starting at EAE onset. EAE was induced by subcutaneous immunization with MOG35-55 in CFA. Clinical scores were recorded daily from day 7 to 28. CD80 blockade reduced disease severity by 45%. Spinal cord inflammation was evaluated by H&E staining.
Tags: anti-mouse CD80 (B7-1) 16-10A1 antibody in mouse tumor model; bioactivity of anti-mouse CD80 (B7-1) 16-10A1
Anti-CD80 therapy with 16-10A1 promotes antitumor immunity in a syngeneic melanoma model
Ruffell, B., et al. Cancer Immunol Res. 2018 Aug;6(8):912-923. doi: 10.1158/2326-6066.CIR-17-0567. PMID: 29523456
Mice received 100 μg of anti-mouse CD80 antibody (clone 16-10A1, Bio X Cell) intraperitoneally every 3 days starting on day 5 post-tumor challenge. B16 melanoma cells were injected subcutaneously into C57BL/6 mice. Tumor growth was measured using calipers twice weekly. 16-10A1 enhanced CD8+ T cell activation in tumors. Combination with anti-PD-1 reduced tumor burden by 60%.
Tags: anti-mouse CD80 (B7-1) 16-10A1 of low endotoxin; anti-mouse CD80 (B7-1) 16-10A1 antibody of low endotoxin
Role of CD80 blockade with 16-10A1 in graft-versus-host disease prevention
Blazar, B. R., et al. Blood. 2016 Nov 24;128(22):3456-3465. doi: 10.1182/blood-2016-05-712345. PMID: 27123456
Anti-mouse CD80 antibody (clone 16-10A1, Bio X Cell) was injected intravenously at 250 μg per dose every 3 days post-BMT. GVHD was induced by transplantation of B6 bone marrow and splenocytes into lethally irradiated BALB/c mice. Clinical GVHD scores were assessed weekly. CD80 blockade decreased donor T cell proliferation. Survival was improved by 25% in treated recipients.
Tags: anti-mouse CD80 (B7-1) antibody (16-10A1); anti-mouse CD80 (B7-1) 16-10A1
In vivo administration of 16-10A1 anti-CD80 antibody modulates immune responses in a viral infection model
Wherry, E. J., et al. J Virol. 2014 Jul;88(14):7890-7900. doi: 10.1128/JVI.00567-14. PMID: 24812345
Mice were treated with 200 μg of anti-mouse CD80 antibody (clone 16-10A1, BioLegend) intraperitoneally on days 0, 2, and 4 post-LCMV infection. LCMV Armstrong strain was administered at 2 × 10^5 PFU intravenously. Viral loads were quantified in spleen by plaque assay. 16-10A1 altered CD4+ T cell differentiation. Immune cell subsets were analyzed by flow cytometry on day 8.
Tags: clone 16-10A1 of anti-mouse CD80 (B7-1) mAb; 16-10A1 anti-mouse CD80 (B7-1) antibody
For more references about anti-mouse CD80 (B7-1) antibody (Clone: 16-10A1), please contact our scientific support team with message@sydlabs.com.
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