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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: Miap301.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD47 monoclonal antibody (clone: Miap301) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: Miap301) specifically binds to mouse CD47.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD47 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD47 monoclonal antibody of clone Miap301 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Single-cell RNA sequencing reveals compartmental remodeling of tumor-infiltrating immune cells induced by anti-CD47 targeting in pancreatic cancer.
Pan, Y., et al. J Hematol Oncol. 2019 Nov 27;12(1):124. doi: 10.1186/s13045-019-0822-6. PMID: 31771616
We investigated if targeting CD47 with a monoclonal antibody could enhance the response of PDAC to ICPi by altering the TME. ...The efficacy of CD47 blockade was examined in xenograft models. CD45+ immune cells from syngeneic tumor models were subjected to single-cell RNA-sequencing (scRNA-seq) by using the 10x Genomics pipeline. ...Our data indicate that CD47 targeting induces compartmental remodeling of tumor-infiltrating immune cells of the TME in PDAC. Different PDAC mouse models exhibited differential response to the anti-CD47 and anti-PD-L1 blockade due to the differential effect of this combination treatment on the infiltrating immune cells and key immune activating genes in the TME established by the different PDAC cell lines. ...Blocking CD47-SIRPα pathway has been shown to be effective in inhibiting several malignancies in preclinical studies. ...Chi-square tests were performed to compare PD-L1, CD47, CD68, and CD163 and clinical features. Spearman’s rank correlation was evaluated to determine the correlation between CD47, CD68, and CD163. ...CD47 expression in human placenta was used as positive control (Fig. 1a).
Tags: anti-mouse CD47 Miap301; anti-mouse CD47 Miap301 mAb
Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo.
Reed, M., et al. Nat Commun. 2019 Nov 1;10(1):5004. doi: 10.1038/s41467-019-12968-y. PMID: 31676794
CD47 is a ubiquitously expressed cell surface glycoprotein that associates with a variety of receptors to facilitate critical cell signaling events. ...However, a lack of tissue-targeted knockout mice has limited studies that address the function of CD47 expression by non-hematopoietic cell types in vivo. ...As CD47 is ubiquitously expressed, understanding contributions of specific CD47-expressing cell types in mucosal wound-healing responses is critical to gain detailed mechanistic insights. ...CD47 expression is absent in the epithelium but retained in the lamina propria and submucosa. Scale bars = 100 μm upper panels, 50 μm lower panels. ...Having established that IEC-targeted Cd47-deficient mice do not develop significant intestinal pathology under baseline conditions, we tested whether epithelial CD47 expression is required for mucosal wound healing.
Tags: anti-mouse CD47 Miap301 in vivo; anti-mouse CD47 Miap301 antibody in vivo
For more references about Anti-mouse CD47 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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