Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: Miap301.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD47 monoclonal antibody (clone: Miap301) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: Miap301) specifically binds to mouse CD47.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD47 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD47 monoclonal antibody of clone Miap301 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Durable antitumor responses to CD47 blockade require adaptive immune stimulation
Sockolosky JT, et al. Science. 2016 May 20;352(6287):816-20. doi: 10.1126/science.aad9480. PMID: 27091975
B16F10 melanoma-bearing mice were treated with anti-mouse CD47 antibody (miap301) i.p. to block CD47 in vivo. Miap301 administration enhanced macrophage phagocytosis of tumor cells in vivo. In vivo miap301 blockade reduced tumor growth in syngeneic models. Combination with PD-L1 antibody amplified miap301 effects on survival. Tumor rechallenge showed memory responses post-miap301 therapy.
Tags: anti-mouse CD47 Miap301; anti-mouse CD47 Miap301 mAb
Anti-CD47 Antibody As a Targeted Therapeutic Agent for Human Lung Cancer and Cancer Stem Cells
Zhang M, et al. Front Immunol. 2017 Apr 20;8:404. doi: 10.3389/fimmu.2017.00404. PMID: 28484448
NOD/SCID mice engrafted with lung cancer cells received anti-mouse CD47 MIAP301 antibody i.p. for in vivo therapy. MIAP301 treatment inhibited tumor growth in xenografts. In vivo MIAP301 enhanced macrophage-mediated clearance of CSCs. Survival was prolonged in MIAP301-treated tumor-bearing mice. No significant toxicity was observed post-MIAP301 administration.
Tags: anti-mouse CD47 Miap301 in vivo; anti-mouse CD47 Miap301 antibody in vivo
CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction
Tseng D, et al. Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18320-5. doi: 10.1073/pnas.1115560108. PMID: 22042861
B16F10 melanoma-bearing mice were injected with anti-mouse CD47 antibody (Miap301) to disrupt SIRPα signaling in vivo. Miap301 treatment potentiated antibody-mediated tumor destruction. In vivo Miap301 blockade reduced melanoma lung metastases. Macrophage infiltration increased in Miap301-treated tumors. Therapeutic efficacy was enhanced by Miap301 in vivo.
Tags: anti-mouse CD47 Miap301 in animal model; anti-mouse CD47 Miap301 mAb in animal model
CD47 blockade triggers T cell-mediated destruction of immunogenic tumors
Liu X, et al. Nat Med. 2015 Aug;21(10):1209-15. doi: 10.1038/nm.3939. PMID: 26238513
Tumor-bearing mice received anti-mouse CD47 antibody (Miap301 clone) i.p. to activate T cells in vivo. Miap301 triggered T cell infiltration into tumors. In vivo Miap301 blockade destroyed immunogenic tumors. Tumor rechallenge resistance developed post-Miap301. CD8+ T cells were essential for Miap301 efficacy in vivo.
Tags: anti-mouse CD47 Miap301 in cancer research; anti-mouse CD47 Miap301 mAb in cancer research
Targeting CD47-SIRPα as an adjunct to tumor immunotherapy
Feng M, et al. Cancer Cell. 2017 Aug 14;32(2):135-137. doi: 10.1016/j.ccell.2017.07.012. PMID: 28723562
MC38 colon carcinoma mice were treated with anti-mouse CD47 (Miap301) to synergize with immunotherapy in vivo. Miap301 enhanced PD-1 blockade antitumor effects. In vivo Miap301 increased dendritic cell cross-presentation. Tumor growth was halted in Miap301 combination therapy. Immune memory was induced by Miap301 in vivo.
Tags: function of anti-mouse CD47 Miap301; anti-mouse CD47 Miap301 mAb of low endotoxin
For more references about anti-mouse CD47 antibody (Clone: Miap301), please contact our scientific support team with message@sydlabs.com.
Copyright © 2009-2025 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.
Friend Link: Ushelf