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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human CD47 Monoclonal Antibodies for Flow Cytometry

Anti-Human CD47 Monoclonal Antibodies for Flow Cytometry

CD47, CD47 antigen, CD47 molecule, CD47 glycoprotein, Integrin associated protein, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6, Rh-related antigen, OA3, IAP

Catalog No. Product Name Size List Price (US$) Quantity
PA007470.m1 Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Mouse IgG1 Kappa 50 ug 60.00
PA007470.m1 Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Mouse IgG1 Kappa 200 ug 120.00
PA007470.h1Fs Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Human IgG1 Fc Silent Kappa 50 ug 120.00
PA007470.h1Fs Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Human IgG1 Fc Silent Kappa 200 ug 240.00
PA007760.h1Fs-b Biotinylated Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Human IgG1 Fc Silent Kappa 50 ug 105.00
PA007760.h1Fs-b Biotinylated Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Human IgG1 Fc Silent Kappa 200 ug 210.00
Description

PA007470.m1: Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Mouse IgG1 Kappa

Recombinant  Mouse IgG1 Monoclonal Antibody. 
Clone: B6H12.
Isotype:  Mouse IgG1 Kappa.
Source: The anti-human CD47 monoclonal antibody (clone: B6H12) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B6H12 antibody specifically binds to human CD47.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007470.h1Fs: Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: B6H12.
Isotype: Human IgG1 kappa.
Source: The anti-human CD47 monoclonal antibody (clone: B6H12) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant B6H12 antibody specifically binds to human CD47.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007760.h1Fs-b: Biotinylated Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain. 
Clone: B6H12.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human CD47 monoclonal antibody (clone: B6H12) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant B6H12 antibody specifically binds to human CD47.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The B6H12 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32)

Kaur A, et al. J Immunol. 2023. PMID: 37737643
This study uses mass spectrometry to explore tapasin-mediated tryptophan editing in HLA-B44 peptidomes. The W6/32 antibody (anti-human HLA-ABC) was purified from mouse ascites and crosslinked to PureProteome Protein A magnetic beads for immunoaffinity purification. Cells were stained with W6/32-FITC antibody for surface HLA class I analysis via quantitative flow cytometry, revealing a significant reduction in HLA-B44:05 molecules on tapasin knockout cells compared to wild-type cells. A slight, non-significant decrease in HLA-B44:05 surface stability was observed in tapasin knockout cells in the presence of brefeldin A.
(Tags: activity of W6/32 antibody; function of W6/32 antibody)
Zhang F, et al. Proc Natl Acad Sci U S A. 2022. PMID: 36136978
This study investigates how sarbecovirus ORF7a proteins inhibit MHC-I antigen presentation. Human 293T, HuH7.5, U2OS, and A549/ACE2 cells were transduced with lentiviral vectors expressing SARS-CoV-2 ORF7a or Nef and stained with the pan-HLA-ABC antibody W6/32 for flow cytometry. MHC-I surface levels, measured by median fluorescence intensity, were reduced in ORF7a-expressing cells. The study compared wild-type and ORF7-deleted SARS-CoV-2 strains, confirming ORF7a's role in suppressing MHC-I expression.
(Tags: W6/32 antibody; W6/32 antibody for cancer research)
Zaitouna AJ, et al. Bio Protoc. 2020. PMID: 33659325
This study evaluates anti-HLA-I monoclonal antibody specificities using solid phase bead arrays. The W6/32 antibody, a pan anti-HLA class I antibody, was used to normalize HLA-I protein levels on Labscreen and FlowPRA Single Antigen Beads. Biotinylated W6/32 was tested at various dilutions (undiluted to 1:100) alongside anti-Bw4 and anti-Bw6 antibodies. Representative data showed W6/32 binding to HLA-I beads, with isotype control IgG2a used for comparison.
(Tags: W6/32 antibody for flow cytometry; W6/32 antibody for mouse tumor model)
Raghavan M, et al. Hum Immunol. 2019. PMID: 30735755
This study describes methods for measuring HLA class I expression and half-lives using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry histograms showed W6/32 signals for monocytes, NK cells, B cells, CD8+ T cells, and CD4+ T cells, with geometric mean values quantifying HLA class I expression. The W6/32 antibody was chosen for its broad recognition of most HLA class I allotypes, facilitating consistent assessment across healthy donors.
(Tags: W6/32 antibody mouse dose range; W6/32 mAb)
Akazawa Y, et al. Cancer Sci. 2019. PMID: 30973665
This study examines HLA class I expression in early-stage hepatocellular carcinoma (HCC) using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry analysis of tissue from five HCC patients compared HLA class I expression between cancer cells and non-tumorous hepatocytes. The mean fluorescence intensity ratio of W6/32 staining indicated higher HLA class I expression in cancer cells, enhancing sensitivity to cytotoxic T lymphocytes. Heterogeneous expression was observed in non-tumorous hepatocytes in one case.
(Tags: W6/32 mAb for flow cytometry; W6/32 mAb for mouse tumor model)
For more technical references or data sheets regarding the Recombinant Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following in vivo grade recombinant anti-human CD47 monoclonal antibodies:
Recombinant Anti-human CD47 monoclonal antibody (Clone: B6H12)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD47 monoclonal antibodies:
Recombinant Anti-mouse CD47 monoclonal antibody (Clone: Miap301)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD370 / CD47 bispecific antibodies:
Recombinant Anti-mouse CD370 / CD47 bispecific antibody (Clone: 10B4 / A4)

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