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Recombinant anti-mouse CD25 mouse IgG2c monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-mouse CD25 monoclonal antibodies are based on the rat monoclonal antibody anti-CD25 (mouse) with the clone number of PC61, PC61.5 or PC61.5.3. The rat IgG1 lamda monoclonal antibody has been validated and reported for use in immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. In vivo depletion of mouse CD25(+)CD4(+)FoxP3(+) Treg cells using the rat PC61 monoclonal antibody is widely used to characterize the Treg function. The PC61 monoclonal antibody specifically binds to an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by the 3C7 and 7D4 monoclonal antibodies. By doing so, the antibody blocks binding of IL-2 to CD25.
The in vivo grade recombinant anti-mouse CD25 mouse IgG2c kappa monoclonal antibody was produced in CHO cells.
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1.
Clone: PC61, PC61.5 or PC61.5.3.
Isotype: mouse IgG2c, kappa.
Applications: immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
The in vivo grade recombinant afucosylated anti-mouse CD25 mouse IgG2c monoclonal antibody was produced in engineered CHO cells which produce afucosylated antibodies.
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1.
Clone: PC61, PC61.5 or PC61.5.3.
Isotype: mouse IgG2c, kappa.
Applications: immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD25 mouse IgG2c kappa monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
1、Regulatory T Cell Depletion Using a CRISPR Fc-Optimized CD25 Antibody
Marit J van Elsas , et al. Int J Mol Sci. 2022 Aug 5;23(15):8707. doi: 10.3390/ijms23158707.PMID: 35955841
The preclinical anti-CD25 (αCD25) antibody, clone PC-61, has met with modest anti-tumor activity due to its capacity to clear Tregs from the circulation and lymph nodes, but not those that reside in the tumor. The optimization of the Fc domain of this antibody clone has been shown to enhance the intratumoral Treg depletion capacity.
Tags: anti-mouse CD25 antibody PC61.5.3; PC61.5.3 anti-mouse CD25 antibody
2、Anti-CD25 monoclonal antibody Fc variants differentially impact regulatory T cells and immune homeostasis.
Huss, D. J., et al. Immunology. 2016 Jul;148(3):276-86. doi: 10.1111/imm.12609. PMID: 27012310
Antibody-mediated targeting of the high-affinity IL-2 receptor α chain (IL-2Rα or CD25) offers a direct mechanism to target IL-2 biology and is being actively explored in the clinic. In mouse models, the rat anti-mouse CD25 clone PC61 has been used extensively to investigate the biology of IL-2 and Treg cells; however, there has been controversy and conflicting data on the exact in vivo mechanistic function of PC61.
Tags: anti-mouse CD25 PC61.5.3 in vivo; anti-mouse CD25 PC61.5.3 mAb in vivo
3、Treg Immunomodulation Contributes to the Anti-atherosclerotic Effects of Huxin Formula in ApoE-/- Mice.
Ou, X. M., et al. Chin J Integr Med. 2024 Oct;30(10):896-905. doi: 10.1007/s11655-024-3663-2. PMID: 38753277
Further, Tregs were depleted by weekly intraperitoneal injection of purified anti-mouse CD25 antibody (PC61, 250 µg per mouse) for 4 weeks in Experiment II (n=6 per group). ...Oil Red O and Masson staining were used to evaluate the plaque area and aortic root fibrosis. ...Quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot were utilized to assess the expression of TGF-β mRNA and protein in the aorta. ...The expression of CD4+T lymphocytes, macrophages and smooth muscle cells in the aortic root were detected by immunofluorescence staining. ...HXF relieved atherosclerosis, probably by restraining inflammatory response, reducing inflammatory cell infiltration and attenuating aortic root fibrosis by increasing Treg counts.
Tags: function of anti-mouse CD25 PC61.5.3; function of anti-mouse CD25 PC61.5.3 mAb
For more references about anti-mouse CD25 antibody (Clone: PC61.5.3) please contact our scientific support team with message@sydlabs.com.
Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
Syd Labs also provides the following research grade antibody biosimilar proteins:
Daclizumab Biosimilar, research grade, Anti-human CD25 Monoclonal Antibody
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