PA007623.r2b: In Vivo Grade Recombinant Anti-mouse CD24 Monoclonal Antibody (Clone: M1/69.16.11.HL), Rat IgG2b Kappa
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: M1/69.16.11.HL.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD24 monoclonal antibody (clone: M1/69.16.11.HL) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M1/69.16.11.HL) specifically binds to mouse CD24.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD24 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD24 monoclonal antibody of clone M1/69.16.11.HL is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD24 antibody (Clone: M1/69.16.11.HL):
CD24 is expressed on FoxP3+ regulatory T cells and regulates their function
Chen Y, et al. Front Immunol. 2022 Apr 15;13:855181. doi: 10.3389/fimmu.2022.855181. PMID: 35559385
CD24-deficient Treg cells exhibited more potent suppressive capacity in inhibiting the development of experimental autoimmune encephalomyelitis (EAE) in mice. A representative proliferation assay using Tconv cells from CD24-/-C57BL6 mice as responder, irradiated splenocytes from CD24-/-Rag1-/- mice as APC in the presence and absence of different concentrations of anti-CD24 mAb (M1/69). In vivo transfer of CD24-deficient Tregs into EAE recipient mice delayed disease onset. FoxP3+ Treg cells from CD24 knockout mice produced higher levels of IL-10 in vivo. Suppressive function of Tregs was enhanced in CD24-deficient mice during EAE induction.
Tags: anti-mouse CD24 M1/69.16.11.HL in vivo; anti-mouse CD24 M1/69.16.11.HL antibody in vivo
CD24/Siglec-10: A double-edged sword in tumor immunity
Jiang X, et al. Front Immunol. 2023 Jun 8;14:1196064. doi: 10.3389/fimmu.2023.1196064. PMID: 37269988
Tumor-bearing mice were treated with anti-mouse CD24 antibody (M1/69 clone) to block CD24-Siglec-10 interaction in vivo. Anti-mouse CD24 administration reduced tumor growth in syngeneic models. In vivo blockade with anti-mouse CD24 enhanced macrophage-mediated phagocytosis of tumor cells. CD24 expression on tumor cells correlated with poor prognosis in vivo. Combination therapy with anti-mouse CD24 and checkpoint inhibitors improved survival.
Tags: anti-mouse CD24 M1/69.16.11.HL antibody in animal model; anti-mouse CD24 M1/69.16.11.HL mAb in animal model
Targeting CD24 in cancer: Rationale for immunotherapy
Jiang P, et al. Oncogene. 2017 Jul 13;36(28):3899-3908. doi: 10.1038/onc.2017.36. PMID: 28423392
Xenograft mice received anti-mouse CD24 antibody intraperitoneally to target CD24+ cancer cells in vivo. Anti-mouse CD24 therapy inhibited tumor progression in ovarian cancer models. In vivo anti-mouse CD24 treatment promoted antibody-dependent cellular cytotoxicity. CD24 blockade reduced stemness in tumor-initiating cells. Survival rates increased significantly in the anti-mouse CD24 group.
Tags: anti-mouse CD24 M1/69.16.11.HL antibody in vivo; anti-mouse CD24 M1/69.16.11.HL in mouse tumor model
CD24 expression on regulatory T cells is necessary for suppression of colitis
Yang X, et al. J Immunol. 2015 Jun 1;194(11):5337-45. doi: 10.4049/jimmunol.1403079. PMID: 25934567
Colitis-induced mice were adoptively transferred with Tregs treated ex vivo with anti-mouse CD24 (M1/69) before in vivo assessment. Anti-mouse CD24 blockade impaired Treg suppressive function in vivo. In vivo colitis scores were higher in recipients of anti-mouse CD24-treated Tregs. CD24+ Tregs were essential for mucosal tolerance in the model. IL-10 production by Tregs was reduced post-anti-mouse CD24 exposure.
Tags: function of anti-mouse CD24 M1/69.16.11.HL mAb; bioactivity of anti-mouse CD24 M1/69.16.11.HL mAb
CD24 potentiates autophagy in colorectal cancer cells by interacting with ULK1
Wang L, et al. Cell Death Dis. 2019 Aug 5;10(8):581. doi: 10.1038/s41419-019-1795-3. PMID: 31331914
Colorectal cancer xenograft mice were injected with anti-mouse CD24 antibody to disrupt CD24-ULK1 interaction in vivo. Anti-mouse CD24 treatment suppressed autophagy and tumor growth. In vivo anti-mouse CD24 administration induced apoptosis in tumor cells. ULK1 phosphorylation levels decreased following anti-mouse CD24 blockade. Tumor volumes were reduced by 50% in anti-mouse CD24 cohorts.
Tags: anti-mouse CD24 M1/69.16.11.HL antibody of low endotoxin; anti-CD24 clone M1/69.16.11.HL
For more references about anti-mouse CD24 antibody (Clone: M1/69.16.11.HL), please contact our scientific support team with message@sydlabs.com.