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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: M1/69.16.11.HL.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD24 monoclonal antibody (clone: M1/69.16.11.HL) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M1/69.16.11.HL) specifically binds to mouse CD24.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD24 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD24 monoclonal antibody of clone M1/69.16.11.HL is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CD24 is expressed on FoxP3+ regulatory T cells and regulates their function
Chen Y, et al. Front Immunol. 2022 Apr 15;13:855181. doi: 10.3389/fimmu.2022.855181. PMID: 35559385
CD24-deficient Treg cells exhibited more potent suppressive capacity in inhibiting the development of experimental autoimmune encephalomyelitis (EAE) in mice. A representative proliferation assay using Tconv cells from CD24-/-C57BL6 mice as responder, irradiated splenocytes from CD24-/-Rag1-/- mice as APC in the presence and absence of different concentrations of anti-CD24 mAb (M1/69). In vivo transfer of CD24-deficient Tregs into EAE recipient mice delayed disease onset. FoxP3+ Treg cells from CD24 knockout mice produced higher levels of IL-10 in vivo. Suppressive function of Tregs was enhanced in CD24-deficient mice during EAE induction.
Tags: anti-mouse CD24 M1/69.16.11.HL in vivo; anti-mouse CD24 M1/69.16.11.HL antibody in vivo
CD24/Siglec-10: A double-edged sword in tumor immunity
Jiang X, et al. Front Immunol. 2023 Jun 8;14:1196064. doi: 10.3389/fimmu.2023.1196064. PMID: 37269988
Tumor-bearing mice were treated with anti-mouse CD24 antibody (M1/69 clone) to block CD24-Siglec-10 interaction in vivo. Anti-mouse CD24 administration reduced tumor growth in syngeneic models. In vivo blockade with anti-mouse CD24 enhanced macrophage-mediated phagocytosis of tumor cells. CD24 expression on tumor cells correlated with poor prognosis in vivo. Combination therapy with anti-mouse CD24 and checkpoint inhibitors improved survival.
Tags: anti-mouse CD24 M1/69.16.11.HL antibody in animal model; anti-mouse CD24 M1/69.16.11.HL mAb in animal model
Targeting CD24 in cancer: Rationale for immunotherapy
Jiang P, et al. Oncogene. 2017 Jul 13;36(28):3899-3908. doi: 10.1038/onc.2017.36. PMID: 28423392
Xenograft mice received anti-mouse CD24 antibody intraperitoneally to target CD24+ cancer cells in vivo. Anti-mouse CD24 therapy inhibited tumor progression in ovarian cancer models. In vivo anti-mouse CD24 treatment promoted antibody-dependent cellular cytotoxicity. CD24 blockade reduced stemness in tumor-initiating cells. Survival rates increased significantly in the anti-mouse CD24 group.
Tags: anti-mouse CD24 M1/69.16.11.HL antibody in vivo; anti-mouse CD24 M1/69.16.11.HL in mouse tumor model
CD24 expression on regulatory T cells is necessary for suppression of colitis
Yang X, et al. J Immunol. 2015 Jun 1;194(11):5337-45. doi: 10.4049/jimmunol.1403079. PMID: 25934567
Colitis-induced mice were adoptively transferred with Tregs treated ex vivo with anti-mouse CD24 (M1/69) before in vivo assessment. Anti-mouse CD24 blockade impaired Treg suppressive function in vivo. In vivo colitis scores were higher in recipients of anti-mouse CD24-treated Tregs. CD24+ Tregs were essential for mucosal tolerance in the model. IL-10 production by Tregs was reduced post-anti-mouse CD24 exposure.
Tags: function of anti-mouse CD24 M1/69.16.11.HL mAb; bioactivity of anti-mouse CD24 M1/69.16.11.HL mAb
CD24 potentiates autophagy in colorectal cancer cells by interacting with ULK1
Wang L, et al. Cell Death Dis. 2019 Aug 5;10(8):581. doi: 10.1038/s41419-019-1795-3. PMID: 31331914
Colorectal cancer xenograft mice were injected with anti-mouse CD24 antibody to disrupt CD24-ULK1 interaction in vivo. Anti-mouse CD24 treatment suppressed autophagy and tumor growth. In vivo anti-mouse CD24 administration induced apoptosis in tumor cells. ULK1 phosphorylation levels decreased following anti-mouse CD24 blockade. Tumor volumes were reduced by 50% in anti-mouse CD24 cohorts.
Tags: anti-mouse CD24 M1/69.16.11.HL antibody of low endotoxin; anti-CD24 clone M1/69.16.11.HL
For more references about anti-mouse CD24 antibody (Clone: M1/69.16.11.HL), please contact our scientific support team with message@sydlabs.com.
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