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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: B3/25.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD71 (TfR1) monoclonal antibody (clone: B3/25) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: B3/25) specifically binds to human CD71 (TfR1).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD71 (TfR1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human CD71 (TfR1) monoclonal antibody of clone B3/25 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Spatial relationships between markers for secretory and endosomal machinery in human cytomegalovirus-infected cells versus those in uninfected cells.
Das, S. et al. J Virol. 2011 Jun;85(12):5864-79. doi: 10.1128/JVI.00155-11. PMID: 21471245
Transferrin receptor (TFRC) (TR; p90; TRFR; transferrin receptor protein 1; CD71; TFR; TFR1; OTTHUMP00000208525; T9). ...Our objective is to understand the process of HCMV virion assembly that takes place in the cytoplasm. ...Studies of cVAC biogenesis and operation will be facilitated by a better understanding of the composition and three-dimensional (3-D) arrangement of the various substructures that form the cVAC. ...As part of developing a more complete structural definition of the cVAC, in this work we have compared in uninfected and infected cells the 3-D locations, extents of colocalization, and intracellular volumes of markers for the ER, Golgi apparatus, early and recycling endosomes, multivesicular bodies, and lysosomes, as well as markers associated with the ESCRT III apparatus. ...Although it was originally thought that viral proteins occlude cellular proteins from the cVAC, work from our and other laboratories has demonstrated that the cVAC consists of components of the secretory apparatus arranged as concentric cylinders.
Tags: anti-human CD71 (TfR1) B3/25; anti-human CD71 (TfR1) B3/25 antibody
Cytotoxic potential of ribonuclease and ribonuclease hybrid proteins.
Rybak, S. M. et al. J Biol Chem. 1991 Nov 5;266(31):21202-7. doi: not provided. PMID: 1939162
Therefore RNase behaves like a potent toxin when directed into a cell. ...To explore the cytotoxic potential of RNase toward mammalian cells, bovine pancreatic ribonuclease A was coupled via a disulfide bond to human transferrin or antibodies to the transferrin receptor. ...The RNase hybrid proteins were cytotoxic to K562 human erythroleukemia cells in vitro with an IC50 around 10(-7) M whereas greater than 10(-5) M native RNase was required to inhibit protein synthesis. ...Cytotoxicity requires both components of the conjugate since excess transferrin or ribonuclease inhibitors added to the medium protected the cells from the transferrin-RNase toxicity. ...After a dose-dependent lag period inactivation of protein synthesis by transferrin-RNase followed a first-order decay constant.
Tags: bioactivity of anti-human CD71 (TfR1) B3/25 mAb; anti-human CD71 (TfR1) B3/25 mAb of low endotoxin
For more references about Anti-human CD71 (TfR1) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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