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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 5E9C11.
Isotype: Mouse IgG1 Kappa.
Source:The anti-human CD71 (TfR) monoclonal antibody (clone: 5E9C11) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 5E9C11) specifically binds to human CD71 (TfR).
Applications:ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD71 (TfR) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human CD71 (TfR) monoclonal antibody of clone 5E9C11 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
RanBPM is a phosphoprotein that associates with the plasma membrane and interacts with the integrin LFA-1.
Denti, S., et al. J Biol Chem. 2004 Mar 26;279(13):13027-13034. doi: 10.1074/jbc.M313515200. PMID: 14722085
Low levels of RanBPM were also detected in immunoprecipitates of several additional transmembrane receptors tested, including CD4, CD71 (transferrin receptor), and MHC class I molecules (Fig. 6A and data not shown). ...The same membranes were reprobed with antibodies against the various immunoprecipitated proteins. Eluates did not contain contaminating LFA-1 proteins, with the exception of the anti-CD71 immunoprecipitates, which reproducibly contained β2 integrins (Fig. 6A). ...Immunoprecipitations were carried out using the following antibodies: anti-LFA-1 (TS1.18 and TS1.22), anti-integrin β1 (polyclonal β1-cyto) (29), anti-CD3 (OKT3), anti-CD4 (S3.5), anti-transferrin receptor (5E9C11), anti-PKCβ I (C-16 sc-209, Santa Cruz Biotechnology). ...To characterize endogenous RanBPM and its interaction with the LFA-1 receptor in mammalian cells, we generated monoclonal antibodies (mAbs) against recombinant RanBPM purified from E. coli. ...Prediction analysis of RanBPM sequence for protein motifs (Scansite Motif Scan, available at scansite.mit.edu/) revealed the presence of putative consensus sites for phosphorylation by serine/threonine kinases, including sites (Ser/Thr-Pro) for proline-directed kinases (31), suggesting that the different migration species observed could be caused by different phosphorylation states of RanBPM.
Tags: anti-human CD71 (TfR) 5E9C11 antibody in animal model; anti-human CD71 (TfR) 5E9C11 mAb in animal model
Attenuation of HLA-DR expression by mononuclear phagocytes infected with Mycobacterium tuberculosis is related to intracellular sequestration of immature class II heterodimers.
Hmama, Z., et al. J Immunol. 1998 Nov 1;161(9):4882-93. doi: 10.4049/jimmunol.161.9.4882. PMID: 9794422
MHC class II expression was examined in macrophages infected with Mycobacterium tuberculosis. IFN-gamma increased the surface expression of class II molecules in THP-1 cells and this was markedly reduced in cells infected with M. ...Metabolic labeling combined with pulse-chase experiments and biochemical analysis showed that the majority of class II molecules in infected cells became resistant to endoglycosidase H, consistent with normal Golgi processing. ...However, results of intracellular staining and dual color confocal microscopy revealed a significant defect in transport of newly synthesized class II molecules through the endocytic compartment. ...Thus, compared with findings in control cells, class II molecules in infected cells colocalized to a minimal extent with a lysosomal-associated membrane protein-1+ endosomal compartment. ....Consistent with defective transport along the endocytic pathway, the maturation of SDS-stable class II alphabeta dimers--dependent upon removal of Ii chain and peptide loading of class II dimers in the MHC class II compartment--was markedly impaired in M.
Tags: anti-human CD71 (TfR) 5E9C11 antibody in cancer research; anti-human CD71 (TfR) 5E9C11 in human tumor model
For more references about Anti-human CD71 (TfR) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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