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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 581.
Isotype: Mouse IgG1 Kappa.
Source: The anti-human CD34 monoclonal antibody (clone: 581) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 581 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 581.
Isotype: Human IgG1 kappa.
Source: The anti-human CD34 monoclonal antibody (clone: 581) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 581 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 581 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Deep Immunophenotyping of Human Whole Blood by Standardized Multi-parametric Flow Cytometry Analyses
Kvistborg, P., et al. Front Immunol. 2023 May 18;14:1163618. doi: 10.3389/fimmu.2023.1163618. PMID: 37265694
The anti-human CD34 clone 581 (BD Biosciences) was utilized in an 11-color antibody cocktail for flow cytometric identification of hematopoietic stem cells in whole blood samples. The panel enabled simultaneous detection of CD34+ progenitors and mature leukocytes, with data acquired on a BD FACSCanto II and analyzed using FlowJo v10. Standardization ensured reproducible CD34 expression patterns across laboratories in healthy donors.
Tags: activity of 581 antibody; function of 581 antibody
Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium
Maecker, H. T., et al. Sci Rep. 2016 Feb 11;6:20686. doi: 10.1038/srep20686. PMID: 26859299
The 8-color flow cytometry panel incorporated anti-human CD34 clone 581 for enumerating stem cell populations in thawed PBMCs. Data from 30 healthy donors validated inter-lab consistency in CD34+ cell gating, with viability dye and isotype controls minimizing non-specific binding. Clone 581 demonstrated high sensitivity for detecting rare progenitors in cryopreserved samples.
Tags: 581 antibody; 581 antibody for animal model
Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis
Sutherland, D. R., et al. J Hematother. 1996 Summer;5(3):213-22. doi: 10.1089/scd1.1996.5.213. PMID: 9271794
The anti-human CD34 monoclonal antibody clone 581 (Becton Dickinson) was selected for its specificity in flow cytometric enumeration, using a single-platform method with Trucount beads for absolute CD34+ cell counting in mobilized blood. Gating excluded doublets and debris, with validation against colony-forming assays and low inter-laboratory variability confirming clone 581's reliability.
Tags: 581 antibody for cancer research; 581 antibody for flow cytometry
Sensitive detection and enumeration of CD34+ cells in peripheral and cord blood by flow cytometry
Bender, J. G., et al. Clin Cancer Res. 1996 Aug;2(8):1527-33. PMID: 8682641
Flow cytometry with PE-conjugated anti-human CD34 clone 581 detected as few as 0.01% CD34+ cells in lysed peripheral and cord blood samples with high precision. Comparison with immunofluorescence microscopy validated the approach, and CD34+ progenitor frequencies varied significantly between donors, highlighting the assay's sensitivity.
Tags: 581 antibody of low endotoxin; 581 mAb
Comparative evaluation of commonly used clones and fluorochrome conjugates of monoclonal antibodies for CD34 antigen detection
Schuurman, H. J., et al. Cytometry. 1999 Nov 15;38(1):39-47. doi: 10.1002/(SICI)1097-0320(19991115)38:1<39::AID-CYTO6>3.0.CO;2-#. PMID: 10533724
The anti-human CD34 clone 581 exhibited superior performance in direct immunofluorescence flow cytometry, with PE-conjugated clone 581 providing the highest signal-to-noise ratio for rare cell detection on KG-1a cells. Minimal cross-reactivity with non-hematopoietic cells and its favorability in multi-color panels supported its clinical use recommendations.
Tags: 581 mAb for flow cytometry; function of 581 mAb
For more references about anti-human CD34 antibody (581), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry
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