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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human CD34 Monoclonal Antibodies for Flow Cytometry

Anti-Human CD34 Monoclonal Antibodies for Flow Cytometry

CD34; CD34 antigen; CD34 molecule

Catalog No. Product Name Size List Price (US$) Quantity
PA007480.m1 Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Mouse IgG1 Kappa 50 ug 55.00
PA007480.m1 Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Mouse IgG1 Kappa 200 ug 110.00
PA007480.h1Fs Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa 50 ug 110.00
PA007480.h1Fs Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa 200 ug 220.00
PA007755.h1Fs-b Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa 50 ug 100.00
PA007755.h1Fs-b Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa 200 ug 200.00
Description

PA007480.m1: Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Mouse IgG1 Kappa

Recombinant  Mouse IgG1 Monoclonal Antibody. 
Clone: 581.
Isotype:  Mouse IgG1 Kappa.
Source: The anti-human CD34 monoclonal antibody (clone: 581) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 581 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007480.h1Fs: Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 581.
Isotype: Human IgG1 kappa.
Source: The anti-human CD34 monoclonal antibody (clone: 581) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 581 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007755.h1Fs-b: Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: 581), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 581.
Isotype:  Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human CD34 monoclonal antibody (clone: 581) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 581 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 581 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Human CD34 Monoclonal Antibody (Clone: 581)

Kvistborg, P., et al. Front Immunol. 2023 May 18;14:1163618. PMID: 37265694
The anti-human CD34 clone 581 (BD Biosciences) was utilized in an 11-color antibody cocktail for flow cytometric identification of hematopoietic stem cells in whole blood samples. The panel enabled simultaneous detection of CD34+ progenitors and mature leukocytes, with data acquired on a BD FACSCanto II and analyzed using FlowJo v10. Standardization ensured reproducible CD34 expression patterns across laboratories in healthy donors.
(Tags: activity of 581 antibody; function of 581 antibody)
Maecker, H. T., et al. Sci Rep. 2016 Feb 11;6:20686. PMID: 26859299
The 8-color flow cytometry panel incorporated anti-human CD34 clone 581 for enumerating stem cell populations in thawed PBMCs. Data from 30 healthy donors validated inter-lab consistency in CD34+ cell gating, with viability dye and isotype controls minimizing non-specific binding. Clone 581 demonstrated high sensitivity for detecting rare progenitors in cryopreserved samples.
(Tags: 581 antibody; 581 antibody for animal model)
Sutherland, D. R., et al. J Hematother. 1996 Summer;5(3):213-22. PMID: 9271794
The anti-human CD34 monoclonal antibody clone 581 (Becton Dickinson) was selected for its specificity in flow cytometric enumeration, using a single-platform method with Trucount beads for absolute CD34+ cell counting in mobilized blood. Gating excluded doublets and debris, with validation against colony-forming assays and low inter-laboratory variability confirming clone 581's reliability.
(Tags: 581 antibody for cancer research; 581 antibody for flow cytometry)
Bender, J. G., et al. Clin Cancer Res. 1996 Aug;2(8):1527-33. PMID: 8682641
Flow cytometry with PE-conjugated anti-human CD34 clone 581 detected as few as 0.01% CD34+ cells in lysed peripheral and cord blood samples with high precision. Comparison with immunofluorescence microscopy validated the approach, and CD34+ progenitor frequencies varied significantly between donors, highlighting the assay's sensitivity.
(Tags: 581 antibody of low endotoxin; 581 mAb)
Schuurman, H. J., et al. Cytometry. 1999 Nov 15;38(1):39-47. PMID: 10533724
The anti-human CD34 clone 581 exhibited superior performance in direct immunofluorescence flow cytometry, with PE-conjugated clone 581 providing the highest signal-to-noise ratio for rare cell detection on KG-1a cells. Minimal cross-reactivity with non-hematopoietic cells and its favorability in multi-color panels supported its clinical use recommendations.
(Tags: 581 mAb for flow cytometry; function of 581 mAb)
For more technical references or data sheets regarding the Recombinant Anti-Human CD34 Monoclonal Antibody (Clone: 581) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry

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