My Cart [ 0 ]
Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human CD34 Monoclonal Antibodies for Flow Cytometry

Anti-Human CD34 Monoclonal Antibodies for Flow Cytometry

CD34; CD34 antigen; CD34 molecule

Catalog No. Product Name Size List Price (US$) Quantity
PA007474.m1 Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Mouse IgG1 Kappa 50 ug 100.00
PA007474.m1 Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Mouse IgG1 Kappa 200 ug 200.00
PA007474.h1Fs Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Human IgG1 Fc Silent Kappa 50 ug 100.00
PA007474.h1Fs Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Human IgG1 Fc Silent Kappa 200 ug 200.00
Description

PA007474.m1: Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Mouse IgG1 Kappa

Recombinant Mouse IgG1 Monoclonal Antibody. 
Clone: My10.
Isotype:  Mouse IgG1 Kappa.
Source: The anti-human CD34 monoclonal antibody (clone: My10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant My10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007474.h1Fs: Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: My10.
Isotype: Human IgG1 kappa.
Source: The anti-human CD34 monoclonal antibody (clone: My10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant My10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The My10 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

References of anti-human CD34 antibody for Flow Cytometry (Clone: My10):


Generation and Characterization of Anti-CD34 Monoclonal Antibodies that React with Hematopoietic Stem Cells
Maleki LA, et al. Cell J. 2014 Spring;15(4):292-300. doi: 10.22074/cellj.2014.532. PMID: 24611141
The My10 monoclonal antibody, recognizing CD34, was tested for specificity via ELISA and Western blotting, with clone 3D5 showing strong reactivity to CD34 peptides and native CD34 from human umbilical cord blood cells. Subisotyping confirmed its immunoglobulin class, and it enabled identification and enrichment of hematopoietic stem/progenitor cells.
Tags: activity of My10 antibody; function of My10 antibody

Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies
Civin CI, et al. Blood. 1987 Nov;70(5):1316-23. doi: 10.1182/blood.V70.5.1316.1316. PMID: 3489673
Two-color cell sorting with My10 and HLA-DR antibodies enriched hematopoietic progenitors in bone marrow, with the My10-positive, HLA-DR-positive fraction containing nearly all CFU-GM and BFU-E. Flow cytometry and progenitor assays confirmed high purity and enrichment of progenitor cells using My10.
Tags: My10 antibody; My10 antibody for animal model

Humanization of an anti-CD34 monoclonal antibody by complementarity-determining region grafting based on computer-assisted molecular modelling
Queen C, et al. Cancer Res. 1992 Dec 15;52(24 Suppl):6761s-6767s. PMID: 1458463
The My10 anti-CD34 antibody was humanized via CDR grafting, retaining binding affinity to CD34+ KG1 cells in flow cytometry. Comparative analysis showed similar reactivity between parental and humanized versions, with molecular modeling guiding framework region selection for humanization.
Tags: My10 antibody for flow cytometry; My10 antibody for human tumor model

Targeting retroviral vectors to CD34-expressing cells: binding to CD34 does not catalyze virus-cell fusion
Kasahara N, et al. Hum Gene Ther. 1999 Mar 20;10(5):937-46. doi: 10.1089/10430349950018625. PMID: 10094198
The My10 scFv, expressed on retroviral vectors, targeted CD34+ KG1 cells, with flow cytometry confirming specific binding. While targeting improved transduction specificity, CD34 engagement did not enhance virus-cell fusion, as assessed in post-binding assays.
Tags: My10 mAb for animal model; My10 mAb for cancer research

My10, a new myeloid cell antigen recognized by a monoclonal antibody
Civin CI, et al. J Exp Med. 1983 Oct 1;158(4):1125-36. doi: 10.1084/jem.158.4.1125. PMID: 6606698
The My10 monoclonal antibody recognized a 115-kD myeloid antigen on blasts and progenitors, absent on mature granulocytes, as shown by flow cytometry. Sorting with My10 enriched CFU-GM, and immunoprecipitation confirmed the antigen’s molecular weight, establishing its utility in myeloid cell identification.
Tags: My10 mAb for human tumor model; My10 monoclonal antibody

For more references about anti-human CD34 antibody (My10), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry

Related Links

See our Privacy Policy