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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: My10.
Isotype: Mouse IgG1 Kappa.
Source: The anti-human CD34 monoclonal antibody (clone: My10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant My10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: My10.
Isotype: Human IgG1 kappa.
Source: The anti-human CD34 monoclonal antibody (clone: My10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant My10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Mouse IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: My10.
Isotype: Mouse IgG1 Kappa.
Source: The site-directed biotinylated anti-human CD34 monoclonal antibody (clone: My10) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant My10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The My10 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Generation and Characterization of Anti-CD34 Monoclonal Antibodies that React with Hematopoietic Stem Cells
Maleki LA, et al. Cell J. 2014 Spring;15(4):292-300. doi: 10.22074/cellj.2014.532. PMID: 24611141
The My10 monoclonal antibody, recognizing CD34, was tested for specificity via ELISA and Western blotting, with clone 3D5 showing strong reactivity to CD34 peptides and native CD34 from human umbilical cord blood cells. Subisotyping confirmed its immunoglobulin class, and it enabled identification and enrichment of hematopoietic stem/progenitor cells.
Tags: activity of My10 antibody; function of My10 antibody
Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies
Civin CI, et al. Blood. 1987 Nov;70(5):1316-23. doi: 10.1182/blood.V70.5.1316.1316. PMID: 3489673
Two-color cell sorting with My10 and HLA-DR antibodies enriched hematopoietic progenitors in bone marrow, with the My10-positive, HLA-DR-positive fraction containing nearly all CFU-GM and BFU-E. Flow cytometry and progenitor assays confirmed high purity and enrichment of progenitor cells using My10.
Tags: My10 antibody; My10 antibody for animal model
Humanization of an anti-CD34 monoclonal antibody by complementarity-determining region grafting based on computer-assisted molecular modelling
Queen C, et al. Cancer Res. 1992 Dec 15;52(24 Suppl):6761s-6767s. PMID: 1458463
The My10 anti-CD34 antibody was humanized via CDR grafting, retaining binding affinity to CD34+ KG1 cells in flow cytometry. Comparative analysis showed similar reactivity between parental and humanized versions, with molecular modeling guiding framework region selection for humanization.
Tags: My10 antibody for flow cytometry; My10 antibody
Targeting retroviral vectors to CD34-expressing cells: binding to CD34 does not catalyze virus-cell fusion
Kasahara N, et al. Hum Gene Ther. 1999 Mar 20;10(5):937-46. doi: 10.1089/10430349950018625. PMID: 10094198
The My10 scFv, expressed on retroviral vectors, targeted CD34+ KG1 cells, with flow cytometry confirming specific binding. While targeting improved transduction specificity, CD34 engagement did not enhance virus-cell fusion, as assessed in post-binding assays.
Tags: My10 mAb for animal model; My10 mAb for cancer research
My10, a new myeloid cell antigen recognized by a monoclonal antibody
Civin CI, et al. J Exp Med. 1983 Oct 1;158(4):1125-36. doi: 10.1084/jem.158.4.1125. PMID: 6606698
The My10 monoclonal antibody recognized a 115-kD myeloid antigen on blasts and progenitors, absent on mature granulocytes, as shown by flow cytometry. Sorting with My10 enriched CFU-GM, and immunoprecipitation confirmed the antigen’s molecular weight, establishing its utility in myeloid cell identification.
Tags: My10 mAb; My10 monoclonal antibody
For more references about anti-human CD34 antibody (My10), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry
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