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Heparinase II Enzyme

Heparin Lyase II; Heparin Eliminase II
Catalog No. Product Name Size List Price (US$) Quantity
BP000032-GD6 Recombinant Heparinase II Enzyme 0.2 IU 480.00
BP000032-GD6 Recombinant Heparinase II Enzyme 0.5 IU 780.00
Description

Heparinase II enzyme cleaves at the 1-4 linkages between hexosamines and uronic acid residues (both glucuronic and iduronic) of heparin and heparan sulfate (relative activity about 1:2), yielding oligoaccharides (mainly disaccharides).

Located in the extracellular matrix, the glycosaminoglycan of heparin plays important physiological roles in anticoagulation and angiogenesis. The acidic polysaccharide of Heparin consists of a heterogeneous disaccharide repeating unit of hexosamine and uronic acid (L-iduronic or D-glucuronic acid) connected through 1-4 linkages and modified with various functional groups.

The three heparinase enzymes (heparinase I, heparinase II, and heparinase III) produced by Flavobacterium heparinum specifically recognize and cleave at different sequences of heparin and heparan sulfate. If interested, please see more information about heparinases and their heparin-related applications.

BP000032-GD6: Recombinant Heparinase II from Flavobacterium heparinum

Source: E. coli-derived
Specific activity >6 IU/mg. One international unit (IU) of recombinant Heparinase II is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.
Molecular weight: 84,100.
Isoelectric point: 8.9 - 9.1.
Form: In hepes buffer with 30% glycerol and 0.01 M Ca2+.

Shipping: Recombinant heparinase II is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.

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