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CRISPR Genome Editing Services

Mammalian Cell Line Engineering Services, CRISPR/Cas9 Gene Editing Services
Catalog No. Product Name Size List Price (US$) Quantity
BS019A Knock-out Cell Line Generation Using CRISPR Genome Editing 1 package Request
BS019B Knock-in Cell Line Generation Using CRISPR Genome Editing 1 package Request
Description

Knock-out and knock-in cell lines are generated by experienced scientists working on lentivirus, adeno-associated virus (AAV), RNAi, and cell line development services for decades. In combination with the powerful CRISPR/Cas9 tool, our proprietary viral system is highly efficient and precise in knock-out and knock-in cell line development with any gene and any host cell, including mammalian primary cell, without introducing foreign DNA.

Generation of knock-out and knock-in cell lines using CRISPR genome editing:

CRISPR/Cas9 DNA constructs are introduced into host cells by viral transduction or non-viral transfection. Knock-out and knock-in cell lines are screened for permanent and precise modification of cellular genome. Our fast and predictive screening techniques may significantly increase speed of knock-out and knock-in cell line development while improving quality and uniformity. The process includes:

- Molecular construction of gRNA, Cas9, and donor vectors based on the target gene/site/locus and cell line specified by clients;
- Virus packaging and production;
- Viral transduction or non-viral transfection of DNA constructs into host cells;
- Selection of cell pool or screening for stable single clones;
- Validation of cell pool or single clones by sequencing and PCR or specific methods such as FACS; (Note: The standard method for validation is sequencing and PCR. Additional fee will be charged for Western blot or cell-based assay of your interest.)
- Batch culture and cell banking.

Service of knock-out cell line generation using CRISPR genome editing includes:

a. Single cell cloning;
b. Detailed clone validation by sequencing;
c. Backup vial storage for 6 months.

Deliverable:
a. The selected clone;
b. Knock-out cell line development study report including gRNA design and sequencing validation results.

Fast turnaround: 16-19 weeks.

Service of knock-in cell line generation using CRISPR genome editing includes:

a. Single cell cloning;
b. Detailed clone validation by sequencing and PCR;
c. Backup vial storage for 6 months.

Deliverable:
a. The selected clone;
b. Knock-in cell line development study report including gRNA design and PCR and sequencing validation results.

Fast turnaround: 20-24 weeks.

Syd Labs also provides the following viral-based cell line generation services:

Lentiviral Based Stable Cell Line Generation Services: development of stable cell lines using lentivirus.
Chimeric Antigen Receptor T-Cell Engineering: development of stable cell lines for precise genomic modification.

Syd Labs also provides the following stable cell line generation services:

Standard Stable Cell Line Development Services: non-viral generation of adhension or suspension stable cell lines with one or two gene insertion.
Stable Cell Line Development Services for Protein Production: development of stable cell lines for recombinant protein expression.
Stable Cell Line Development Services for Antibody Production: development of stable cell lines for recombinant antibody expression.
Stable Cell Line Development Services for Biologics Production: generation of stable cell lines for manufacturing recombinant protein or antibody drugs.

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