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Conditions of optimal VEGFR2 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation.
Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occuring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
BP000995-PKA-242: Recombinant Human VEGFR2
Source:Insect Cells-derived.
A disulfide-linked homodimeric, glycosylated, polypeptide containing 757 amino acids and having a molecular mass of 160 kDa.
The activity of VEGFR2 D1-7 was determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surfaces receptors. The ED50 for this effect is typically 10.0 ng/ml, corresponding to a specific activity of 100,000 IU/mg.
Purity > 95%, by SDS-PAGE.
Formulation: KDR was lyophilized from a concentrated (1 ug/ml) sterile solution containing 25 mM MES pH-5.5 and 100 mM NaCl.
Reconstitution: It is recommended to reconstitute the lyophilized VEGFR2 in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other VEGFR2 Proteins:
Recombinant Human VEGFR2 Fc Chimera
VEGFR2 Antibodies:
VEGFR2 (Ab-951) Polyclonal Antibody
VEGFR2 Monoclonal Antibody
VEGFR2 (Ab-1214) Polyclonal Antibody
VEGFR2 (Ab-1054) Polyclonal Antibody
VEGFR2 (Ab-1059) Polyclonal Antibody
VEGFR2 (Ab-1175) Polyclonal Antibody
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