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Conditions of optimal VEGF-E Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
A DNA sequence encoding the mature variant of VEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes.
BP000259-CYT-263:Recombinant Human VEGF-E (Orf Virus)
Source: Escherichia Coli-derived.
The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF-E Orf Virus has been determined to be in the range of 5-20 ug/ml corresponding to a Specific Activity of 50,000-200,000 IU/mg.
Purity: > 90.0% as determined by RP-HPLC and SDS-PAGE.
Formulation: The protein was lyophilized from a concentrated (1 ug/ml) solution containing PBS pH-7.4.
Reconstitution: The lyophilized VEGF-E is soluble in water and most aqueous buffers. The lyophilized Vascular Endothelial Growth Factor-E (Orf Virus) should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 ug/ml.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
(1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.)
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