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EK000115-EK0849: Mouse TrkB ELISA Kit
Range 93.8ug/ml-6000ug/ml
Sensitivity < 5 ug/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of mouse TrkB in tissue lysates or cell culture supernates.
The significance of detection in sera, plasma, body fluids is undetermined.
Expiration: four months at 4°C and eight months at -20°C.
Background
TrkB is the high affinity catalytic receptor for several "neurotrophins", BDNF (Brain Derived Neurotrophic Factor), NT-4 (neurotrophin-4), and NT-3 (neurotrophin-3), which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases, including a sub-family of protein kinases which includes TrkA and TrkC. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. The standard product used in this kit is recombinant TrkB, a 49.5KDa glycoprotein, C32?439.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Nakagawara A, Liu XG, Ikegaki N, White PS, Yamashiro DJ, Nycum LM, Biegel JA, Brodeur GM (January 1995). "Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)". Genomics 25 (2): 538?6.
2. Soppet, D., Escandon, E., Maragos, J., Middlemas, D. S., Reid, S. W., Blair, J., Burton, L. E., Stanton, B. R., Kaplan, D. R., Hunter, T., Nikolics, K., Parada, L. F. The neurotrophic factors brain-derived.neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor. Cell 65: 895-903, 1991.
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