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Conditions of optimal SNAP25 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Introduction
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters.
BP002041-PRO-396: Recombinant C.elegans Synaptosomal-associated 25 kDa protein (SNAP25)
Source: E. coli-derived.
Recombinant C.elegans Synaptosomal-associated 25 kDa protein (SNAP25) is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23 kDa.
SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. Recombinant C.elegans Synaptosomal-associated 25 kDa protein (SNAP25) was purified by using conventional chromatography techniques.
Purity: > 95.0% as determined by SDS-PAGE.
Formulation: The protein contains 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM DTT, 1 mM EDTA and 10% Glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Other SNAP25 Protein:
Recombinant Human SNAP25 protein
SNAP25 Antibodies:
SNAP25 Polyclonal Antibody
SNAP25 Polyclonal Antibody
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