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Conditions of optimal SARS-Spike antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
PA005182-ANT-157: SARS-Spike Polyclonal Antibody
Rabbit Polyclonal Antibody.
Applications: This polyclonal antibody can be used for detection of SARS Spike protein in Western blot. Full-length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139KDa band is detected. Western blot analysis of Spike in (A) transfected and (B) untransfected mouse melanoma cell lysate at a concentration of 0.05 ug/ml.
Immunogen: The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 1124-1140 of the putative SARS Spike glycoprotein (Genbank accession no. NP_828851).
Form of Antibody: 100ugs in 200ul PBS containing 0.2% gelatin and 0.05% sodium azide.
Isotype: Rabbit IgG.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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