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BP003348: Rat IL-22R alpha 1 Protein with the C-His Tag
Source: The recombinant Rat IL-22R alpha 1 Protein, (amino acids His16-Ala228) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Rat IL-22R alpha 1 protein with the His tag at the C-terminus has a predicted MW of 25.42 kDa. Due to glycosylation, the protein migrates to 37-45 kDa based on Tris-Bis PAGE result.
Activity: The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Rat IL-22R alpha 1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP003313: Rat IL-22R alpha 1 Protein with the C-hFc Tag
Source: The recombinant Rat IL-22R alpha 1 Protein, (amino acids His16-Ala228) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Rat IL-22R alpha 1 protein with the hFc tag at the C-terminus has a predicted MW of 51.08 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human IL-22 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Rat IL-22R alpha 1 protein with the C- hFc Tag with the EC50 of 30.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Rat IL-22R alpha 1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Rat IL-22R alpha 1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following IL-22R Protein :
Biotinylated Human IL-22R alpha 1&IL-10R beta Protein
Cynomolgus IL-22R alpha 1 Protein
Human IL-22R alpha 1 Protein
Human IL-22R alpha 1&IL-10R beta Protein
Mouse IL-22R alpha 1 Protein
Rat IL-22R alpha 1 Protein
Syd Labs provides the following IL-22 Protein :
Biotinylated Mouse IL-22 Protein
Cynomolgus IL-22 Protein
Human IL-22 Protein
Recombinant Mouse IL-22 Protein
IL-22 Protein
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