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Conditions of optimal Phospho Crebtide antibody performance should be determined experimentally by the investigator.
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Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Description
Crebtide (KRREILSRRPSYR) is a substrate peptide derived from protein Creb, which is widely utilized as a synthetic substrate for PKA and PKC kinase family. Affinity purified rabbit polyclonal anti-pCrebtide is developed with phosphorylated Crebtide (KRREILSRRPpSYR) and absorbed with non-phosphorylated Crebtide (KRREILSRRPSYR). This antibody has been successful utilized to develop the non-radioactive assay for protein kinase using Crebtide as substrate.
PA000593-P0182: HRP-conjugated phospho-Crebtide Polyclonal Antibody
Rabbit Polyclonal Antibody.
Specificity/Sensitivity: Recognize only the phophophorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay, not cross-react with the non-phosphorylated Crebtide
Applications: ELISA (1:1000), WB (1:1000).
Immunogen: phospho-Crebtide (KRREILSRRPpSYR)-KLH.
Purification: Affinity purified with KRREILSRRPpSYR on Agarose followed by affinity absorption with non-phosphorylated Crebtide.
Form of Antibody: 250 umg/mL in PBS, pH7.0, 50% glycerol.
Storage/Stability: Stable for 1 year at -20°C and 3 months at -20°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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