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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal NANOG Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
BP002381-PRO-090: Recombinant Human Homeobox protein NANOG-TAT (NANOG)
Source: E. coli-derived.
Recombinant Human NANOG protein is a single, non-glycosylated, Polypeptide chain containing 305 amino acids and having a molecular mass of 34.6 kDa. The NANOG protein is fused to an N-terminal TAT (transcriptional activator protein) tag and purified by proprietary chromatographic techniques.
N-terminal sequence analysis: Met-Gly-Arg-Lys-Lys.
Purity: > 95.0% as determined by SEC-HPLC and SDS-PAGE.
Formulation: NANOG was lyophilized after extensive dialysis against PBS.
Reconstitution: It is recommended to reconstitute the lyophilized NANOG in sterile PBS not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other NANOG Proteins:
Recombinant Human Homeobox protein NANOG
Recombinant Human Homeobox protein NANOG, His Tag
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