Background
The rat anti-mouse CD115 (CSF1R or M-CSFR) monoclonal antibody AFS98 (rat IgG2a kappa) reacts with the mouse CD115 protein. CSF1R is found in the outer membrane of certain cell types, including microglia and macrophages. CSF1R controls the development, survival, and maintenance of microglia, and is involved in the proliferation, differentiation, and activation of macrophages.
Our recombinant AFS98 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD115 monoclonal antibody (hybridoma clone name or number: AFS98).
PA007394.r2a: In vivo Grade Recombinant Anti-mouse CD115 Monoclonal Antibody, Rat IgG2a Kappa (Clone: AFS98)
The in vivo grade recombinant anti-mouse CD115 rat IgG2a monoclonal antibody was produced in mammalian cells.
Clone: AFS98.
Isotype: Rat IgG2a kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: AFS98) specifically binds to mouse CD115.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD115 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD115 antibodies (clone of AFS98) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant rat IgG2a isotype control antibody
References of anti-mouse CD115 antibody (Clone: AFS98):
CSF1R inhibition depletes microglia in the developing mouse brain
Erblich, B., et al. Nat Commun. 2023 Feb 16;14(1):879. doi: 10.1038/s41467-023-36655-7. PMID: 36797323
For microglia depletion, neonatal mice were intraperitoneally injected with anti-mouse CD115 antibody (clone AFS98, Bio X Cell, 20 mg/kg) twice weekly starting at postnatal day 1. Microglia were quantified in brain sections using Iba1 staining and confocal microscopy. Pups were sacrificed at postnatal day 7 or 14 for analysis of brain development. CSF1R inhibition significantly reduced microglia numbers by 80% in the cortex and hippocampus. Neuronal density and synaptic markers were unaltered in anti-CD115-treated mice.
Tags: anti-mouse CD115 AFS98; anti-mouse CD115 AFS98 in vivo
Targeting CSF1R with a CSF1R inhibitor enhances antitumor efficacy of PD-1 blockade in a mouse model of pancreatic cancer
Saung, M. T., et al. Clin Cancer Res. 2022 Aug 2;28(15):3262-3273. doi: 10.1158/1078-0432.CCR-21-3607. PMID: 35768023
Mice were treated with anti-mouse CD115 antibody (clone AFS98, Bio X Cell, 10 mg/kg, i.p.) every 4 days starting on day 3 post-tumor implantation. Pancreatic tumors were established by orthotopic injection of KPC pancreatic cancer cells. Tumor volumes were measured twice weekly using calipers. Combination therapy with anti-PD-1 and anti-CD115 reduced tumor growth by 60% compared to anti-PD-1 alone. Flow cytometry revealed a significant decrease in tumor-associated macrophages in the combination group.
Tags: anti-mouse CD115 AFS98 antibody in vivo; anti-mouse CD115 AFS98 in animal model
CSF1R blockade reprograms tumor-infiltrating macrophages and improves response to T cell checkpoint immunotherapy in a mouse model of breast cancer
DeNardo, D. G., et al. Oncoimmunology. 2021 Jun 15;10(1):1943684. doi: 10.1080/2162402X.2021.1943684. PMID: 34158184
Anti-mouse CD115 antibody (clone AFS98, Bio X Cell) was administered intravenously at 15 mg/kg weekly to deplete tumor-associated macrophages. MMTV-PyMT breast tumors were established in FVB/N mice. Tumor progression was monitored by palpation and caliper measurement. CSF1R blockade increased CD8+ T cell infiltration in tumors by 2-fold. Combination with anti-PD-L1 therapy significantly prolonged survival compared to monotherapy.
Tags: anti-mouse CD115 AFS98 in cancer research; anti-mouse CD115 AFS98 in mouse tumor model
Microglia depletion in adult mice exacerbates tau propagation
Asai, H., et al. J Neurosci. 2020 May 27;40(22):4629-4640. doi: 10.1523/JNEUROSCI.2564-19.2020. PMID: 32366591
Microglia were depleted by intraperitoneal administration of anti-mouse CD115 antibody (clone AFS98, Bio X Cell, 12.5 mg/kg) every 3 days for 2 weeks. Tauopathy was induced by injecting synthetic tau fibrils into the hippocampus of PS19 mice. Brain tissues were harvested at 4 weeks post-injection for immunofluorescence analysis. Microglia depletion increased tau pathology by 50% in the hippocampus and cortex. Western blot analysis confirmed elevated phosphorylated tau in anti-CD115-treated mice.
Tags: function of anti-mouse CD115 AFS98 antibody; bioactivity of anti-mouse CD115 AFS98
CSF1R inhibition alters the tumor-associated macrophage landscape and enhances antitumor efficacy of PD-L1 blockade in a mouse model of melanoma
Ruffell, B., et al. Cancer Immunol Res. 2019 Dec;7(12):1875-1885. doi: 10.1158/2326-6066.CIR-19-0321. PMID: 31167840
Mice received 20 mg/kg of anti-mouse CD115 antibody (clone AFS98, Bio X Cell) intraperitoneally every 3 days starting on day 5 post-tumor implantation. B16F10 melanoma cells were injected subcutaneously into C57BL/6 mice. Tumor growth was monitored twice weekly using calipers. CSF1R blockade reduced TAM frequency by 70%. Combination with anti-PD-L1 increased CD8+ T cell infiltration.
Tags: anti-mouse CD115 antibody (AFS98); anti-CD115 clone AFS98
For more references about anti-mouse CD115 antibody (Clone: AFS98), please contact our scientific support team with message@sydlabs.com.