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Conditions of optimal MUG Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
E. coli Mismatch Uracil DNA Glycosylase (Mug protein), also known as G/U mismatch-specific DNA glycosylase, is an 18 kDa constitutively expressed protein which belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. Uracil bases in DNA can arise from deamination of cytosine giving rise to increased spontaneous mutations. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.
BP001310-C152: Recombinant E.coli Mismatch-specific uracil DNA-glycosylase (MUG) Enzyme
Source: E. coli-derived.
Recombinant E.coli Mismatch-specific uracil DNA-glycosylase (MUG) Enzyme is expressed with sequence (Met1-Arg168) of E.coli Mug.
Predicted molecular mass: 18 kDa.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Formulation: 0.2 um filtered solution of 20 mM Tris-HCl, 2.5 mM -ME, 1 mM PMSF, 50% Glycerol, pH 8.0.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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