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EK000108-EK0763: Mouse GALECTIN-1 ELISA Kit
Range: 156ug/ml-10,000ug/ml
Sensitivity: < 5ug/ml
Specificity : No detectable cross-reactivity with any other cytokine.
Application:For quantitative detection of mouse GALECTIN-1 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.[1][2]The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.[3]Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse.[4] They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. ^ Gitt MA, Barondes SH (Feb 1991). "Genomic sequence and organization of two members of a human lectin gene family". Biochemistry 30 (1): 82?.
2. ^ Gauthier L, Rossi B, Roux F, Termine E, Schiff C (Oct 2002). "Galectin-1 is a stromal cell ligand of the pre-B cell receptor (BCR) implicated in synapse formation between pre-B and stromal cells and in pre-BCR triggering". Proc Natl Acad Sci U S A 99 (20): 13014?.
3. Baldini, A., Gress, T., Patel, K., Muresu, R., Chiariotti, L., Williamson, P., Boyd, Y., Casciano, I., Wells, V., Bruni, C. B., Mallucci, L., Siniscalco, M. Mapping on human and mouse chromosomes of the gene for the beta-galactoside-binding protein, an autocrine-negative growth factor. Genomics 15: 216-218, 1993.
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